Search Results (147)

Functionally graded materials (FGMs) show continuous variations in properties and offer unique multifunctional capabilities. This study presents a simulation of the powder bed fusion (PBF) process for FGM fabrication using a combination of Unity-based deposition and lattice Boltzmann method (LBM)-based process models. The study introduces a diffusion model that allows for the simulation of material mixtures, in particular AISI 316L austenitic steel and 18Ni maraging 300 martensitic steel. The Unity-based model simulates particle deposition with controlled distribution, incorporating variations in particle size, friction coefficient, and chamber wall rotation angles. The LBM model that simulated free-surface fluid flow, heat flow, melting, and solidification during the PBF process was extended with diffusion models for mixture fraction and concentration-dependent properties. Comparison of the results obtained in simulation with the experimental data shows that they are consistent. Future research may be connected with further verification and validation of the model, by modeling different materials. The presented model can be used for the simulation, study, modeling, and optimization of the production of functionally graded materials in PBF processes. Full article

The viscoelastic properties of biological membranes are crucial in controlling cellular functions and are determined primarily by the lipids’ composition and structure. This work studies these properties by varying the structure of the constituting lipids in order to influence their interaction with high-density lipoprotein (HDL) particles. Various fluorescence-based techniques were applied to study lipid domains, membrane order, and the overall lateral as well as the molecule–internal glycerol region mobility in HDL–membrane interactions (i.e., binding and/or cargo transfer). The analysis of interactions with HDL particles and various lipid phases revealed that both fully fluid and some gel-phase lipids preferentially interact with HDL particles, although differences were observed in protein binding and cargo exchange. Both interactions were reduced with ordered lipid mixtures containing cholesterol. To investigate the mechanism, membranes were prepared from single-lipid components, enabling step-by-step modification of the lipid building blocks. On a biophysical level, the different mixtures displayed varying stiffness, fluidity, and hydrogen bond network changes. Increased glycerol mobility and a strengthened hydrogen bond network enhanced anchoring interactions, while fluid membranes with a reduced water network facilitated cargo transfer. In summary, the data indicate that different lipid classes are involved depending on the type of interaction, whether anchoring or cargo transfer. Full article

In this paper, we present a microfluidic flow cytometer for simultaneous imaging and dielectric characterization of individual biological cells within a flow. Utilizing a combination of dielectrophoresis (DEP) and high-speed imaging, this system offers a dual-modality approach to analyze both cell morphology and dielectric properties, enhancing the ability to analyze, characterize, and discriminate cells in a heterogeneous population. A high-speed camera is used to capture images of and track multiple cells in real-time as they flow through a microfluidic channel. A wide channel is used, enabling analysis of many cells in parallel. A coplanar electrode array perpendicular to cell flow is incorporated at the bottom of the channel to perform dielectrophoresis-based dielectric characterization. A frequency-dependent voltage applied to the array produces a non-uniform electric field, translating cells to higher or lower velocity depending on their dielectric polarizability. In this paper, we demonstrate how cell size, obtained by optical imaging, and DEP response, obtained by particle tracking, can be used to discriminate viable and non-viable Chinese hamster ovary cells in a heterogeneous cell culture. Multiphysics electrostatic-fluid dynamics simulation is used to develop a relationship between cell incoming velocity, differential velocity, size, and the cell’s polarizability, which can subsequently be used to evaluate its physiological state. Measurement of a mixture of polystyrene microspheres is used to evaluate the accuracy of the cytometer. Full article

Granular systems, no matter whether they are dry or saturated, are commonly encountered in both natural scenarios and engineering applications. In this work, we tackle the compaction problem of both dry and saturated granular systems under gyratory shearing compaction, where particles are subjected to constant pressure and continuous shear rate, which is quite different from the traditional cyclic shearing compaction. Such phenomena are crucial to the compaction of asphalt mixtures or soils in civil engineering and can be extended to other areas, such as powder processing and pharmaceutical engineering. In this study, we investigated the behavior of both dry and fully saturated mono-dispersed granular materials under gyratory shearing compaction using the discrete element method (DEM) and found that the gyratory speed or interstitial fluid viscosity has almost no impact on the compaction behavior, while the pressure and the particle size play more important roles. Additionally, it is the inertial time scale which dictates the compaction behavior under gyratory shearing in most cases; meanwhile, the viscous time scale can also have influence in some conditions. This work determines the similarity and unity between the granular gyratory compaction and the rheology of granular systems, which has direct relevance to various natural and engineering systems. Full article

Exposure to air pollution poses a risk to human respiratory health, and a preventive and therapeutic remedy against fine dust-induced respiratory disease is needed. Background/Objectives: The respiratory-protective effects of Lysimachia mauritiana (LM) against airway inflammation were evaluated in a mouse model exposed to a fine dust mixture of diesel exhaust particles and particulate matter with a diameter of less than 10 µm (PM10D). Methods: To induce airway inflammation, PM10D was intranasally injected into BALB/c mice three times a day for 12 days, and LM extracts were given orally once per day. The immune cell subtypes, histopathology, and expression of inflammatory mediators were analyzed from the bronchoalveolar lavage fluid (BALF) and lungs. Results: LM alleviated the accumulation of neutrophils and the number of inflammatory cells in the lungs and the BALF of the PM10D-exposed mice. LM also reduced the release of inflammatory mediators (MIP-2, IL-17, IL-1α, CXCL1, TNF-α, MUC5AC, and TRP receptor channels) in the BALF and lungs. Lung histopathology was used to examine airway inflammation and the accumulation of collagen fibers and inflammatory cells after PM10D exposure and showed that LM administration improved this inflammation. Furthermore, LM extract inhibited the MAPK and NF-κB signaling pathway in the lungs and improved expectoration activity through an increase in phenol red release from the trachea. Conclusions: LM alleviated PM10D-exposed neutrophilic airway inflammation by suppressing MAPK/NF-κB activation. This study indicates that LM extract may be an effective therapeutic agent against inflammatory respiratory diseases. Full article

Under atmospheric pressure, partial discharge initiated by free metallic particles has consistently been a significant factor leading to failures in high-voltage electrical equipment. Simulating the propagation of negative streamer discharge in N₂/O₂ mixtures contributes to a better understanding of the occurrence and evolution of partial discharge, optimizing the insulation performance of electrical equipment. In this study, a two-dimensional plasma fluid dynamics model coupled with the current module was employed to simulate the evolution process of negative streamer discharge caused by one free metallic particle under a suspended potential at 220 kV applied voltage conditions. Simulation results indicated that the discharge process could be divided into two distinct stages: In the first stage, the electron ionization region detached from the electrode surface and propagated independently. During this stage, the corona discharge on the negative electrode surface provided seed electrons crucial for the subsequent development of negative corona discharge. The applied electric field played a dominant role in the propagation of the electron region, especially in the electron avalanche region. In the second stage, space charge gradually took over, causing distortion in the spatial field, particularly generating a substantial electric field gradient near the negative electrode surface, forming an ionization pattern dominated by ionization near the negative electrode surface. These simulation results contribute to a comprehensive understanding of the complex dynamic process of negative streamer discharge initiated by free metallic particles, providing essential insights for optimizing the design of electrical equipment and insulation systems. Full article

1,2-propylene glycol and its 50-50 w/w mixture with water were used to prepare heat transfer fluids based on amino-functionalized silica. On top of pH-neutral dispersions (no reagents added except for the solvent and the particles), dispersions acidified with acetic acid and with HCl were used to enhance the positive electric charge of silica particles. The colloidal particles had a positive zeta potential >40 mV and showed apparent particle radii of 70 nm, and these properties remained unchanged on heating up to 80 °C for up to 28 days. Full article

Micro-corona devices could be extensively utilized in gas sensing, switchgear, biomedicine, and other fields. As the influence mechanism of the cathode curvature radius on micro-corona discharge dynamical processes is very important for performance optimization and the promotion of these devices, a micro-scale corona discharge gas model in a mixture of N₂-O₂ is proposed based on the fluid–chemical mixing method, which describes the dynamic process of the discharge at atmosphere and normal temperatures. To reveal the influence mechanism of the nanowire curvature radius on the micro-corona discharge, the effect of the cathode nanowire radius on the discharge current, electric field, ionization reaction rate, and charged particle characteristics at different gaps and voltages were determined. The findings indicate that the effect of curvature radius on discharge intensity varies under different gap and voltage conditions. Further analysis indicates that an increase in curvature radius reduces the electric field near the tip while increasing the ionization area and secondary emission area as well as the number of positive ions in the space, consequently affecting the coupling process between the collision ionization and the secondary emission. Especially under the conditions of either small gap or low voltage, a suitable increase in the curvature radius could promote the coupling process and then increase the discharge current. Full article

Lab-on-a-chip technology has been developed to streamline biochemical experiments by providing experimental environments in microscopic areas. Due to the difficulty of mixing chemicals in such small channels, various micromixers have been created. Our proposed sidewall-driven micromixer offers easy fabrication and precise control over mixing concentrations. In our previous study, we successfully generated concentration gradients by simultaneously mixing multiple chambers using a single actuator. However, it is not possible to mix different chemicals in each chamber. In this study, we developed a sidewall-driven micromixer that enables independent mixing in each chamber by installing one actuator per chamber. Experimental results showed that different conditions were achieved in each chamber using both microbead-mixture water and colored water. Thus, this mixer can be used to manipulate concentrations regardless of whether the mixing targets are particles or fluids. Full article

Methanol synthesis from CO₂ can be made in the presence of a sorbent to increase the achievable yield. If the fresh sorbent is continuously fed to a fluidized bed and separated from the catalyst bed by segregation, a steady-state operation can be achieved. The objective of the present work is to provide insight on the suitable operating conditions for such a fluidized bed reactor system. For this, a conventional CuO/ZnO/Al₂O₃ was selected as the catalyst, and the SiOLITE^® zeolite was selected as the sorbent. Different particle sizes were used to be tested in various proportions to perform the fluidized bed segregation study. The fluid dynamics and segregation of the catalyst–sorbent binary mixtures were the most critical points in the development of this proof of concept. A good bed segregation with a mixing index of 0.31 was achieved. This fact favors the correct operation of the system with the continuous addition of adsorbent, which had hardly any catalyst losses during the tests carried out, achieving a loss of 0.005 g/min under optimal conditions. Continuous feeding and removal of sorbent with a low loss of catalyst was observed. Reactor simulations with MATLAB provided promising results, indicating that the addition of sorbent considerably improves the methanol yield under some operating conditions. This makes it more viable for industrial scaling, since it allows us to considerably reduce the pressure used in the methanol synthesis process or to increase the yield per step, reducing the recirculation of unconverted reactants. Full article

Controlling the distribution of the abrasive grains on the surface of the grinding tools in an appropriate way is important for improving the quality of grinding processing and meeting the workpiece precision requirements. In the present study, a novel method for the orderly arrangement of abrasive particles is proposed by using the liquid film flow on the surface of the rotating disk as the driving and controlling means for the uniform dispersion and position arrangement of abrasive particles. Computational fluid dynamics (CFD) simulations have been performed to clearly illustrate the trajectories of abrasive particles under the strong influence of liquid film flow on the rotating disk and reveal the effects of fluid flow, disk rotational motion, and the mixture viscosity on the particle distribution. A new abrasive grain arrangement device is designed and fabricated using this novel method. The operating parameters such as liquid volume flow rate, disk rotational speed, and liquid viscosity are adjusted to control the placement of abrasive grains on the surface of the grinding tool. An image processing tool is used to examine and analyze the arrangement results. The experimental results indicated that the application of the liquid film flow on a rotating disk to the abrasive grain arrangement can improve the arrangement of abrasive grains and get rid of the dependence on the template. Full article

This paper presents the continuation of experimental investigations conducted by the present authors to measure and compare the thermal and fluid dynamic performance of a residential hydronic air coil using nanofluids. The prior experiments were limited to testing only one volumetric concentration (1%) of aluminum oxide (Al₂O₃) nanofluid. They compared it with the base fluid, a 60% ethylene glycol/40% water mixture by mass (60% EG). The original tests revealed some deficiencies in the experimental setup, which was subsequently revised and improved. This paper summarizes the results of experiments from the improved test bed using three concentrations of Al₂O₃ nanofluids: 1, 2, and 3% volumetric concentrations prepared with an average particle size of 45 nm in a 60% EG dispersion. The test bed in these experiments simulates a small air handling system typical of heating, ventilation, and air conditioning (HVAC) applications in cold regions. Entering conditions for the air and liquid were selected to emulate typical commercial air handling systems operating in cold climates. Contrary to previous findings, our test results revealed that nanofluids did not perform as well as expected. Prior predictions from many analytical and numerical studies had promised significant performance gain. The performance of the 1% nanofluid was generally equal to that of the base fluid under identical inlet conditions. However, the performance of the 2% and 3% nanofluids was considerably lower than that of the base fluid. The higher concentration nanofluids exhibited heat rates up to 14.6% lower than the 60% EG and up to 44.3% lower heat transfer coefficient. The 1% Al₂O₃/60% EG exhibited a 100% higher pressure drop across the coil than the base fluid, considering equal heat output. This performance degradation was attributed to the inability to maintain nanofluid dispersion stability, agglomeration, and subsequent decline in the thermophysical properties. Full article

This article focuses principally on the comparison baseline and the optimized flow efficiency of the final stage of an axial turbine operating on a gas–steam mixture by applying a hybrid Nelder–Mead and the particle swarm optimization method. Optimization algorithms are combined with CFD calculations to determine the flowpaths and thermodynamic parameters. The working fluid in this study is a mixture of steam and gas produced in a wet combustion chamber, therefore the new turbine type is currently undergoing theoretical research. The purpose of this work is to redesign and examine the last stage of the gas–steam turbine’s flow characteristics. Among the optimized variables, there are parameters characterizing the shape of the endwall contours within the rotor domain. The values of the maximized objective function, which is the isentropic efficiency of the turbine stage, are found from the 3D RANS computation of the flowpath geometry changing during the improvement scheme. The optimization process allows the stage efficiency to be increased by almost 4 percentage points. To achieve high-quality results, a mesh of over 20 million elements is used, where the percentage error in efficiency between the previous and current mesh sizes drops below 0.05%. Full article

A chimeric protein, formed by two fragments of the conserved nucleocapsid (N) and S2 proteins from SARS-CoV-2, was obtained as a recombinant construct in Escherichia coli. The N fragment belongs to the C-terminal domain whereas the S2 fragment spans the fibre structure in the post-fusion conformation of the spike protein. The resultant protein, named S2NDH, was able to form spherical particles of 10 nm, which forms aggregates upon mixture with the CpG ODN-39M. Both preparations were recognized by positive COVID-19 human sera. The S2NDH + ODN-39M formulation administered by the intranasal route resulted highly immunogenic in Balb/c mice. It induced cross-reactive anti-N humoral immunity in both sera and bronchoalveolar fluids, under a Th1 pattern. The cell-mediated immunity (CMI) was also broad, with positive response even against the N protein of SARS-CoV-1. However, neither neutralizing antibodies (NAb) nor CMI against the S2 region were obtained. As alternative, the RBD protein was included in the formulation as inducer of NAb. Upon evaluation in mice by the intranasal route, a clear adjuvant effect was detected for the S2NDH + ODN-39M preparation over RBD. High levels of NAb were induced against SARS-CoV-2 and SARS-CoV-1. The bivalent formulation S2NDH + ODN-39M + RBD, administered by the intranasal route, constitutes an attractive proposal as booster vaccine of sarbecovirus scope. Full article

Archaeosomes were manufactured from natural archaeal lipids by a microfluidics-assisted single-step production method utilizing a mixture of di- and tetraether lipids extracted from Sulfolobus acidocaldarius. The primary aim of this study was to investigate the exceptional stability of archaeosomes as potential carriers for oral drug delivery, with a focus on powdered formulations. The archaeosomes were negatively charged with a size of approximately 100 nm and a low polydispersity index. To assess their suitability for oral delivery, the archaeosomes were loaded with two model drugs: calcein, a fluorescent compound, and insulin, a peptide hormone. The archaeosomes demonstrated high stability in simulated intestinal fluids, with only 5% of the encapsulated compounds being released after 24 h, regardless of the presence of degrading enzymes or extremely acidic pH values such as those found in the stomach. In a co-culture cell model system mimicking the intestinal barrier, the archaeosomes showed strong adhesion to the cell membranes, facilitating a slow release of contents. The archaeosomes were loaded with insulin in a single-step procedure achieving an encapsulation efficiency of approximately 35%. These particles have been exposed to extreme manufacturing temperatures during freeze-drying and spray-drying processes, demonstrating remarkable resilience under these harsh conditions. The fabrication of stable dry powder formulations of archaeosomes represents a promising advancement toward the development of solid dosage forms for oral delivery of biological drugs. Full article