Search Results (2,715)

Background: Magnolol (MG) and honokiol (HK) are bioactive compounds extracted from Magnolia obovata and Magnolia Officinalis trees with significant pharmacological properties, including antioxidant and antibacterial activity. However, their poor water solubility and low bioavailability limit the therapeutic potential. Methods: To address these limitations, this study aims to develop MG and HK formulations by co-electrospinning using custom-synthesized β-cyclodextrin–oligolactide (β-CDLA) derivatives. MALDI MS and NMR were employed for the structural assessment of the β-CDLA derivatives. This polymer-free electrospinning technique utilizes the high solubility of β-CDLA to incorporate MG and HK into fibrous webs. The morphology of the resulting fibers is established by SEM and further characterized using FTIR and NMR spectroscopy to confirm the successful incorporation of MG and HK. The antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, while the antimicrobial activity was evaluated against several standard microorganisms (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans). Results: The MG and HK electrospun formulations were prepared using highly concentrated feed solutions in dimethylformamide (180% w/v). The resulting β-CDLA fibers, with diameters above 400 nm and an active compound content of 7% wt., exhibited enhanced long-term antioxidant activity and improved antimicrobial efficacy, including notable activity against Escherichia coli. Conclusions: This study demonstrates the potential of MG and HK-loaded β-CDLA fibrous formulations as delivery systems with prolonged antioxidant activity and notable antibacterial efficacy, providing a promising platform for biomedical applications. Full article

The nutritional value of honey is closely related to its components, which are highly influenced by the botanic origin. C. pi. Shen (Codonopsis pilosula (Franch.) var. modesta (Nannf.) L. T. Shen), a key plant in traditional Chinese medicine, mainly produced in Gansu Province of China, has attracted much attention for its medicinal value. However, there are few reports about C. pi. Shen honey. Therefore, this study aimed to evaluate the contents of phenolic profiles and antioxidant activities in C. pi. Shen honey by colorimetric, UPLC-MS/MS, Ferric ion Reducing Antioxidant Power (FRAP), 2,2-bisazo-bis (3-ethyl-benzothiazole-6-sulfonic acid) (ABTS) free radical capacity, and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) scavenging ability methods. In comparison with four other high-yield unifloral honeys in China (Acacia honey, linden honey, rape honey, and jujube honey), C. pi. Shen honey demonstrated remarkable advantages. Specifically, the levels of total phenolic acids, total flavonoids, and phenolic components of C. pi. Shen honey were significantly pronounced, especially protocatechuic acid and kaempferol. Furthermore, the antioxidant activity of C. pi. Shen honey was also excellent, which was attributed to its phenolic compounds, underscoring its potential biological value. This study is anticipated to serve as a reference for the identification, nutritional assessment, and functional advancement of C. pi. Shen honey. Full article

In recent years, the invasive Atlantic blue crab (Callinectes sapidus) has increased its spread throughout the Mediterranean Sea, threatening native biodiversity and local economies. This study aimed to valorize C. sapidus sampled in Sicily by utilizing its exoskeleton as a source of chitosan, astaxanthin, and bio-phenolic compounds. These biomolecules were evaluated for their reducing, radical scavenging, and antitumor activity. The ferric ion reducing antioxidant power (FRAP) and the free radical scavenging activity against radical 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were significantly higher for chitosan (3.16 ± 0.10 mg AAE/g and 8.1 ± 0.10 µmol TE/g). No significant differences were observed among the tested biomolecules in their activity in scavenging the radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Both bio-phenolic compounds and astaxanthin exhibited dose-dependent cytotoxicity on CaCo-2 (IC₅₀ = 12.47 and 18 µg/mL) and HepG2 (IC₅₀ = 10.25 and 1.26 µg/mL) cell lines, while only bio-phenols showed no cytotoxic effect on differentiated CaCo-2 cells up to 20 µg/mL. These findings highlight the value of blue crab by-products in supporting a circular economy, offering a sustainable approach to managing this invasive species while providing bioactive compounds with promising medical and nutraceutical applications. Full article

In this study, we aimed to investigate the inhibitory effects of cumin and cumin extracts from different origins (Hami, Turpan, and Hetian) on the formation of β-carboline heterocyclic amines (HCAs) in smoked meat and simulated systems, and to assess their potential as natural inhibitors in the food industry. The novelty of our research lies in the comprehensive comparative analysis of cumin extracts from different origins, which has not been fully explored in previous studies. We first conducted a quantitative analysis of the total phenol and flavonoid content in cumin extracts from the three origins and evaluated their antioxidant capacities. Subsequently, through simulation experiments, we assessed the inhibitory effects of these extracts on the formation of β-carboline heterocyclic amines and determined their free radical scavenging abilities. To further validate the practical application potential of these extracts, we prepared meat patty samples containing different concentrations of cumin powder, simulating actual processing conditions. The experimental results showed that while the total phenol content in cumin extracts from all origins was similar, averaging around 1.56 mg/g, there was a significant difference in the total flavonoid content, with the highest level observed in the Hetian cumin extract at 6.7 mg/g. Additionally, the Hetian cumin extract demonstrated superior antioxidant capacity, with an FRAP antioxidant activity reaching 21.04 μM TE/g dw, the highest among all samples. Our study also found that the inhibitory effect of cumin extracts on HCA formation was closely related to their free radical scavenging ability, with the Hetian cumin extract showing the strongest scavenging capacity. The addition of cumin powder to meat patties significantly reduced the content of β-carboline heterocyclic amines, particularly at lower cumin concentrations. In summary, our research results highlight the potential of cumin, especially from Hetian, as a natural inhibitor of β-carboline heterocyclic amine formation in processed meats. This study not only provides the food industry with a potential natural additive to improve food safety and quality, but also offers new directions for future research, namely by comparing natural plant extracts from different origins to explore their potential applications in food processing. Full article

To improve the high-value application of millet bran, a water-soluble polysaccharide was extracted from fermented millet bran (FMBP) by using Bacillus natto fermentation. A neutral polysaccharide, FMBP-1, was separated and purified from FMBP using an anion exchange column. Its structure and antioxidant activity in vitro were characterized and determined. The molecular weight of FMBP-1 was 1.154 × 10⁴ Da, and its molecular weight distribution was relatively uniform. The monosaccharide composition, FT-IR, methylation, and NMR results indicated that FMBP-1 was only composed of glucose and was an α-(1→4)-D-glucan that branched at O-6 with a terminal 1-linked α-D-Glcp as a side chain. In addition, the antioxidant assays indicated that FMBP-1 possessed certain capacities for scavenging free radicals and reducing power, and this was in a concentration-dependent manner. This research will provide fundamental data regarding the structure–activity relationship of millet bran polysaccharides and provide a theoretical foundation for the high-value utilization of millet bran within the food and pharmaceutical industries. Full article

Red currant (Ribes rubrum L.) berries are rich in bioactive compounds and exhibit nutritive and protective features. This research examined the content of secondary metabolites of the red currant variety Redpoll lyophilized juice and waste extract and their antioxidative and spasmolytic effects. The flavonol and anthocyanin contents of the samples were determined using high-performance liquid chromatography. The antispasmodic effects were assessed in in vitro conditions, and the potential impact of the samples and possible action mechanisms were revealed. The results showed the prevalence of cyanidin-3-O-glucoside as the dominant anthocyanin with higher content in the juice sample. Quercetin content, as the prevalent flavonol, was higher in the waste sample. The berry juice showed a greater ability for scavenging free radicals, whereas the waste better inhibited lipid peroxidation. The juice was a superior antispasmodic agent for spontaneous, KCl-, CaCl₂-, BaCl₂-, histamine-, and acetylcholine-induced ileal contractions. This first evaluation of the red currant variety Redpoll lyophilized juice and waste extract indicated the beneficial effects of juice as an antioxidant and spasmolytic agent. Therefore, this red currant juice sample could be designated for the prevention or treatment of gastrointestinal disorders. Full article

Background: The genus Astragalus is renowned for its diverse bioactive potential, yet the chemical composition and biological properties of Astragalus melanophrurius remain inadequately explored. This study aimed to investigate the chemical profile, antioxidant capacity, and enzyme inhibitory activities of methanol extracts from various plant parts of A. melanophrurius. Methods: Methanol extracts were obtained from leaves, stems, flowers, roots, and aerial portions of A. melanophrurius. The chemical composition was determined using LC–ESI–MS/MS, focusing on key phytochemicals such as hyperoside, kaempferol, 4-hydroxybenzoic acid, and chlorogenic acid. Antioxidant activities were assessed via DPPH, ABTS, and FRAP assays, while enzyme inhibitory activities were evaluated against α-amylase and tyrosinase. In silico molecular docking analyses were conducted to explore the interactions between major compounds and target enzymes. Results: The leaf extract exhibited the highest total phenolic and flavonoid contents, correlating with superior antioxidant activities, achieving IC₅₀ values of 16.55 mg/mL, 4.58 mg/mL, and 3.07 mg/mL in DPPH, ABTS, and FRAP assays, respectively. The root extract demonstrated notable α-amylase (IC₅₀ = 2.99 mg/mL) and tyrosinase (IC₅₀ = 1.34 mg/mL) inhibitory activities, suggesting potential applications in diabetes and hyperpigmentation management. Molecular docking revealed stable complexes of hyperoside and kaempferol with target enzymes, supporting their roles in observed bioactivities. Conclusions: This study highlights the bioactivity of A. melanophrurius extracts, particularly from leaves and roots, supporting their therapeutic potential. Future research should focus on isolating active compounds and conducting in vivo studies to confirm efficacy and elucidate mechanisms of action. Full article

(1) Background: The RoXsta^TM system has been developed as a rapid, effective means of profiling different types of antioxidant activity. The purpose of this study was to examine its performance utilizing a diverse array of biological fluids including semen, blood plasma, serum, urine, saliva, follicular fluid and plant extracts. (2) Methods: The RoXsta^TM system was used to assess the ability of different fluids to suppress free radical formation as well as scavenge a variety of toxic oxygen metabolites including free radicals and both hydrogen and organic peroxides. (3) Results: Human semen was shown to have significantly (p < 0.001) more peroxide scavenging power than any other fluid tested (10–14 mM vitamin C equivalent compared with 1–2 mM for blood serum or plasma), while urine was particularly effective in scavenging free radicals and preventing free radical formation (p < 0.001). The powerful antioxidant properties of human semen were shown to reside within the seminal plasma (SP) fraction, rather than the spermatozoa, and to be resistant to snap freezing in liquid nitrogen. Moreover, comparative studies demonstrated that human SP exhibited significantly (p < 0.001) higher levels of antioxidant potential than any other species examined (stallion, bull, dog) and that this intense activity reflected the relative vulnerability of human spermatozoa to peroxide attack. (4) Conclusions: The RoXsta^TM system provides valuable information on the antioxidant profile of complex biological fluids, supporting its diagnostic role in conditions associated with oxidative stress. Based on the results secured in this study, human semen is identified as a particularly rich source of antioxidants capable of scavenging both hydrogen and organic peroxides, in keeping with the high susceptibility of human spermatozoa to peroxide-mediated damage. Full article

Beneficial activities of phenolic compounds in the gastrointestinal tract, such as antiradical activity, are affected by the food matrix. The aim of this study was to investigate the influence of one constituent of the food matrix (dietary fiber β-glucan) on the release and antiradical activity of phenolic compounds from apples in gastrointestinal digestion. Simulated digestion in vitro was conducted on whole apples without or with added β-glucan. Antiradical activity was determined with the DPPH method. The total amount of released phenolic compounds in the stomach (563 mg kg⁻¹ fresh weight (fw), 85%) decreased in the intestine (314 mg kg⁻¹ fw, 47%) (p < 0.05). The presence of β-glucan decreased the release of phenolic compounds to 80 and 74% in the stomach and to 44 and 40% in the small intestine when there were lower and higher β-glucan amounts, respectively. A statistical analysis showed differences between release in digestion without or with β-glucan. B-glucan adsorbed up to 24 (stomach) and 32 mg g⁻¹ (small intestine) of the phenolics. Phenolic compounds scavenged more free radicals in the small intestine than in the stomach, and β-glucan decreased this activity, but not significantly. The interaction between β-glucan and phenolic compounds should be considered when explaining the beneficial effects in the stomach and small intestine. Full article

Honey is abundant in bioactive compounds, which demonstrate considerable therapeutic effects, particularly on oxidative stress and inflammation. Objectives: This work sought to evaluate the antioxidant mechanisms of Manuka honey (MH) and Ohia Lehua honey (OLH), correlating them with phytochemical analyses in a rat model of experimentally induced inflammation. Methods: The identification of polyphenolic compounds in the extracts was carried out using HPLC-ESI MS. The extracts’ antioxidant activity was evaluated in vitro through DPPH, FRAP, H₂O₂, and NO scavenging assays, while in vivo assessments included measurements of total oxidative status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), advanced oxidation protein products (AOPP), malondialdehyde (MDA), nitric oxide (NO), and total thiols (SH). Results: The phytochemical analysis found a rich content of phenolic compounds in MH and lower quantities in OLH. In terms of in vitro activity, both MH and OLH exhibited strong DPPH radical scavenging abilities, effective NO and H₂O₂ scavenging capacities, and high FRAP-reducing power. In vivo, OLH proved highly effective in enhancing antioxidant capacity and lowering oxidative stress markers, showing significant increases in TAC and substantial reductions in TOS and OSI levels. Conversely, MH displayed limited and dose-dependent antioxidant activity, a considerable increase in TAC and SH, and a moderate decrease in TOS and OSI levels. Conclusions: To our knowledge, this is the first study to assess the phenolic content of OLH and to show its capacity to scavenge free radicals and reduce oxidative stress. The effectiveness of MH primarily relies on its increased antioxidant properties and depends on concentration. These results highlight the importance of investigating natural products in developing antioxidant strategies. Full article

This paper describes the total antioxidant capacity (TAC) of sweet lupin (Lupinus luteus cv. Lord—LLL) and narrow-leaved bitter lupin (Lupinus angustifolius cv. Mirela—LAM) sprouts fermented by yeast, determined by deploying the updated analytical strategy based on three assays. The procedures covered electrochemical, spectrophotometric, and photochemiluminescence methods. Cyclic voltammetry (CV), the scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH⦁), and photochemiluminescence (PCL) assays against superoxide anion radicals were applied to hydrophilic (ACW) and lipophilic (ACL) fractions, and the data obtained were used to calculate the TAC after sequence extraction of the samples with 80% methanol followed by methanol/hexane (4:1; v/v). The total polyphenol content (TPC) in the hydrophilic fractions was measured using Folin–Ciocalteu reagent. The fermentation of the LLL and LAM seeds had no impact on the antioxidant capacity of their H fractions, whereas it increased the content of their L fractions up to 56%. The germinated lupin seeds of both cultivars showed slightly increased TAC than the fermented ones. The TPC in the yeast-fermented sprouts was slightly higher compared to the LLL sprouts, about twofold higher than in the fermented LLL seeds, and finally almost fivefold higher compared to the LLL seeds. A beneficial effect of fermentation was found with respect to LAM materials. The TAC of the fermented LLL and LAM sprouts measured via CV and PCL assays was almost twofold higher and eight and six times higher, respectively, compared to the seeds. These findings clearly suggest that the seeds of yellow sweet lupin (Lupinus luteus cv. Lord) are the most suitable for producing fermented sprouts with a high content of electroactive polar compounds able to scavenge multiple free radicals of biological and non-biological origin. The updated analytical strategy for the determination of the total antioxidant capacity proved to be a viable tool for screening processed lupin seeds. Full article

The aim of this study was to compare the antioxidant potential in the fruits of different hybrids of Rubus idaeus and Rubus occidentalis (four hybrids) against the fruit of known cultivars of both species (R. idaeus—three cultivars; R. occidentalis—five cultivars) and, using chemometric analysis, to select factors affecting the level of polyphenols and antioxidant properties. Antioxidant activity was determined using the ABTS, DPPH and FRAP tests. Chemometric analysis enabled the separation of R. idaeus and R. occidentalis cultivars and classified the hybrid R. idaeus/R. occidentalis R1314701 as belonging to the R. occidentalis species. Moreover, two hybrids, Rubus occidentalis/Rubus idaeus R1613411 and R. idaeus/R. occidentalis R1613409, can be classified as a purple raspberry. Crossbreeding species/cultivars of the Rubus genus may result in an increased content of anthocyanins, but on the other hand, it may lead to a reduction in free radical scavenging activity in the ABTS and DPPH. Spearman’s correlations confirm the correlations between the total polyphenol content and antioxidant activity in the DPPH, ABTS and FRAP, as well as the anthocyanin content and antioxidant activity in the ABTS and FRAP tests. Chemometric analysis can be an effective tool in determining the species affiliation of obtained hybrids and cultivars. Full article

Recently, there has been a growing demand for plant-based products to treat a range of health conditions. Platostoma menthoides (L.), a member of the Lamiaceae family, is widely known for its versatile therapeutic properties. The primary aim of this study is to analyze and identify the secondary metabolites found in the extract of P. menthoides obtained using ethyl acetate and to assess its antioxidant, antimicrobial, and mosquito larvicidal properties for the first time. For the chemical profiling, a GC–MS analysis of the extract was conducted, and it showed the presence of various phytoconstituents, and the FT-IR spectrum revealed the functional groups associated with them. The quantitative phytochemical estimations revealed values of 34.87 ± 0.53 mg of GAE equivalents/g and 22.19 ± 1.11 mg of QUE equivalents/g, respectively, for total phenolic and flavonoid content. The biological studies of P. menthoides extract showed potent mosquito larvicidal activity against fourth instar larvae of Aedes aegypti at a 100 µg/mL concentration with LC₅₀ and LC₉₀ values of 81.328 and 161.471 µg/mL, respectively. Moreover, the DPPH and FRAP radical scavenging potentials were evaluated, and the results revealed their ability to scavenge the free radicals. The extract also showed higher antibacterial effects against gram-negative bacteria when compared to gram-positive bacteria. All these findings suggest that P. menthoides is a rich source of phytoconstituents with various medicinal applications and can be used as an antioxidant, antibacterial, and mosquito-larvicidal agent. Full article

With the growing consumer demand for natural functional ingredients that promote health and well-being while preventing age-related diseases, this study aimed to develop extruded snacks enriched with Curcuma longa (turmeric) microcapsules, recognized for their significant antioxidant properties. Unripe banana flour (Musa ABB cv. Kluai ‘Namwa’) and rice (Oryza sativa) flour were employed as a gluten-free base to create this novel extruded snack. Curcuma longa extract microcapsules were prepared using a spray-drying technique with varying core-to-wall ratios. Antioxidant capacities were assessed through DPPH, ABTS, superoxide radical scavenging, metal chelating, and ferric-reducing assays. The CM6 microcapsules, prepared at 140 °C with a 1:10 core-to-wall ratio, exhibited potent antioxidant activity, with 58.93 ± 3.31% inhibition for DPPH radicals, 87.58 ± 1.33% for ABTS, and 78.41 ± 1.40% for superoxide radicals. Snacks enriched with 0.25% CM6 microcapsules received high consumer acceptance, with an average liking score of 7.5 out of 9. These findings suggest that snacks made with these gluten-free flours and Curcuma longa microcapsules could be novel, convenient, and appealing functional food products that offer an attractive way to deliver antioxidant benefits with high consumer acceptance. Further research on evaluating the active constituents in the snack, its long-term health benefits, and shelf-life stability is recommended for commercialization. Full article

Since the imbalance between free radicals and antioxidants in the body plays a significant role in the physiology of common, often dangerous diseases, an emphasis is placed on enriching the daily diet with compounds characterized by antioxidant activity. Good sources of natural antioxidants are bee products such as honey, bee pollen, bee bread and propolis, and the best path for introducing the latter products into the diet is mixing them with honey. However, the characteristics of bee product mixtures are not yet fully understood. Therefore, the aim of this study is to verify the health-promoting properties of a mixture of honeydew honey and multifloral bee bread. The profile of phenolic compounds, radical scavenging activity, total phenolic content, diastase number, and also proline and HMF content were determined. The obtained results indicated the improved health-promoting value of this mixture, as increases in radical scavenging activity (from 82.7 to 88.4%), in the total content of phenolic compounds (from 74.6 to 118.8 mg·100 g⁻¹), and also in the proline content (from 64.0 to 95.5 mg·100 g⁻¹) and diastase activity (from 22.6 to 38.8 Schade units) were observed when 5% of bee bread (w/w) was added. Moreover, the bee bread addition provided two important flavonoids to the honeydew honey, i.e., rutin and kaempferol. Full article