Search Results (183,683)

This presented research examined the wall paintings in the Church-Ossuary Presentation of the Blessed Virgin, part of the most important Rila Monastery complex in Bulgaria, painted by the painters from Mount Athos in 1795. The painting materials used to create the unique murals were studied for the first time by optical microscopy (OM), attenuated total reflectance Fourier transform infrared (ATR–FTIR), scanning electron microscopy energy dispersive X-ray spectroscopy (SEM–EDS), and enzyme-linked immunosorbent assay (ELISA). The vibrational and elemental analysis showed that the color palette of the paintings is composed of pigments traditional for Orthodox church wall paintings such as natural pigments, including yellow ochre, red ochre, green earth, and calcite, as well as other historical pigments of synthetic origin, including smalt, red lead, cinnabar, and verdigris. The analysis of the binders by the ATR–FTIR spectroscopy and enzyme-linked immunosorbent assay (ELISA) analysis implied the use of the post-Byzantine egg tempera technique. Only the blue backgrounds in the murals were painted using a smalt-based paint mixed with a carbohydrate binder. Based on the current analysis and comparison with the successive paintings in the other churches of the Rila Monastery, it could be concluded that the technology of the painting process followed by the Athonite artists during the work in the Church-Ossuary became a point of reference for many Bulgarian icon-painters later. Full article

Low-cost enzyme production is considered a feasibility factor in enzyme commercialization. Okara, a high-nutritional agro-industrial residue from soybean processing, was performed as a medium for bacterial amylase production to save costs and increase productivity. This study aimed to produce, characterize, activate amylase, and evaluate the material cost for media from okara. Under solid-state fermentation (SSF) of okara without pretreatment, Bacillus subtilis J12 could produce 983 U/g of amylase within 24 h. Bacillus subtilis J12 amylase had optimal activity at pH 6.0 and 50 °C and was stable at a moderate temperature for up to 120 min. Identified as a metalloenzyme, the activity was improved by ferric ions. The purification of amylase resulted in two fractions which contained at least two types of amylases. Compared with other producers, the production was evaluated using low-cost media without additional supplementations. Based on the productivity, characteristics, and evaluation, Bacillus subtilis J12 amylase was potentially commercialized, had economic value, possessed energy-saving features, and could be applied for industrial use. Full article

Despite the great success in the global exploration and development of reef reservoirs, research on bioclastic shoals under reefs is still in its infancy. Bioclastic shoal reservoirs are very thin, with multiple vertical levels and fast lateral changes, which makes accurate prediction of the reservoir’s location much tougher. To further implement the reservoir distribution, under the guidance of sequence stratigraphy, the prediction of thin shoals under the control of an isochronous stratigraphic framework was established. Using the combination of spectrum shaping and F-X domain noise suppression techniques and utilizing the signal-to-noise ratio spectrum set as the reference, logging curve as supervision, and well seismic calibration and isochronal amplitude slicing as quality control, the seismic frequency band was extended, and the seismic data resolution and signal-to-noise ratio were improved. After frequency extension, the global optimal seismic automatic interpretation technique was used to construct an isochronal stratigraphic framework model. Through waveform facies-controlled inversion and waveform facies-controlled simulation techniques, the elastic properties of the shoal reservoir were obtained, from which the planar distribution of the reservoir was accurately predicted. The above methods were applied to the prediction of the bioclastic shoal reservoir in the lower submember of the Changxing formation in the Yuanba gas field (China). The plane distribution of bioclastic shoal in the first and second levels was identified, which provides a guideline for the prediction of thin shoal reservoirs. Full article

The hydrogen evolution reaction (HER) in the renewable energy system has gained a lot of attention from researchers as hydrogen is assumed to be a clean and renewable carrier. Transition metals and their compounds have been used as promising alternatives to precious noble metals for the HER, offering low cost, more availability, and high activity. In this work, we discussed the mechanisms of the HER and how morphology influenced the catalytic performance of transition metal disulfide (TMD), focusing on structures that range from zero-dimensional (0D) to three-dimensional (3D) TMD materials. Notably, two-dimensional (2D) TMDs, like nanosheets, exhibit the lowest overpotential and a very small Tafel slope, which can be ascribed to their inherent layered structure and large surface area. According to recent research reports, the efficacy and efficiency of the HER process are influenced by surface chemistry, electrochemical characteristics, and the existence of active sites. Full article

Green energy consumption is a pressing global environmental issue that necessitates the optimization of food system circularity to effectively utilize natural resources. The rise of food technology has introduced upcycled food as a promising solution for reducing food waste and promoting sustainability. Despite its potential, research on consumer attitudes toward upcycled food remains scarce. This study investigates the role of upcycled food in shaping Taiwanese consumers’ behaviors in response to environmental challenges, utilizing the value–attitude–behavior (VAB) model with a focus on variables such as “product novelty”, “green perceived value”, and “ethical consumption”. Using convenience sampling, 297 valid responses were collected, achieving a response rate of 91.1%. Findings indicate that health values significantly enhance positive attitudes toward upcycled food, which in turn positively influences behavioral intentions. However, neither green perceived value nor product novelty significantly affected these intentions. These insights highlight the importance of prioritizing health values, consumer attitudes, and ethical consumption in marketing strategies for upcycled food to engage potential consumers and promote environmental sustainability. This study addresses a critical gap in the existing literature and suggests a strategic direction for the food industry. Full article

In Bandpass Sampling Fourier Transform Spectroscopy, a comprehensive method for evaluating signal-to-noise ratio (SNR) has not yet been established. This paper employs an energy conservation approach to analyze the relationship of SNR between interferogram and spectra in Bandpass Sampling Time-Modulated Fourier Transform Spectroscopy (BPS-FTS). It systematically presents models for the average SNR of the system interferogram and the average SNR of reconstructed spectra under different parameters. These models are compared with SNR models in traditional Fourier transform spectroscopy (FTS) and are mutually validated through theoretical modeling and simulation analysis. It provides a theoretical basis and simulation verification for the design and implementation of the system. Full article

Multibeam echosounders provide ideal data for the semi-automated seabed feature extraction and accurate morphometric measurements. In this study, bathymetric and raw backscatter data were initially used to manually delimit the reef morphologies found in an insular semi-enclosed gulf in the northern Aegean Sea (Gera Gulf, Lesvos Island, Greece). The complexity of this environment makes it an ideal area to “crash test” (test to the limit) and compare the results of the delineation methods. A large number of (more than 7000) small but prominent reefs were detected, which made manual mapping extremely time-consuming. Three semi-automated tools were also employed to map the reefs: the Benthic Terrain Modeler (BTM), Confined Morphologies Mapping (CoMMa), and eCognition Multiresolution Segmentation. BTM did not function properly with irregular reef footprints, but by modifying both the bathymetry and slope, the outcome was improved, producing accurate results that appeared to exceed the accuracy of manual mapping. CoMMa, a new GIS morphometric toolbox, was a “one-stop shop” that, besides generating satisfactory reef delineation results (i.e., detecting the same total reef area as the manual method), was also used to extract the morphometric characteristics of the polygons resulting from all the methods. Lastly, the Multiresolution Segmentation also gave satisfactory results with the highest precision. To compare the final maps with the distribution of the reefs, mapcurves were created to estimate the goodness-of-fit (GOF) with the Precision, Recall, and F1 Scores producing values higher than 0.78, suggesting a good detection accuracy for the semi-automated methods. The analysis reveals that the semi-automated methods provided more efficient results in comparison with the time-consuming manual mapping. Overall, for this case study, the modification of the bathymetry and slope enabled the results’ accuracy to be further enhanced. This study asserts that the use of semi-automated mapping is an effective method for delineating the geomorphometry of intricate relief and serves as a powerful tool for habitat mapping and decision-making. Full article

The natural polyphenol resveratrol is a biologically active compound that interacts with DNA and affects the activity of some nuclear enzymes. Its effect on the interaction between nucleosomes and poly(ADP-ribose) polymerase-1 (PARP1) and on the catalytic activity of PARP1 was studied using Western blotting, spectrophotometry, electrophoretic mobility shift assay, and single particle Förster resonance energy transfer microscopy. Resveratrol inhibited PARP1 activity at micro- and sub-micromolar concentrations, but the inhibitory effect decreased at higher concentrations due to the aggregation of the polyphenol. The inhibition of PARP1 by resveratrol was accompanied by its binding to the enzyme catalytic center and a subsequent decrease in PARP1 affinity to nucleosomal DNA. Concurrent binding of talazoparib to the substrate binding pocket of PARP1, which occurs in the presence of resveratrol, restores the interaction of PARP1 with nucleosomes, suggesting that the binding sites of resveratrol and talazoparib overlap. The data suggest that resveratrol can be classified as a natural inhibitor of PARP1. Full article

Conventional mineral fertilization (CMF) is a common practice in infertile sugarcane-cultivated tropical soils, increasing production costs and environmental concerns. Combining CMF with composted sewage sludge (CSS) could be a sustainable strategy. We aim to evaluate changes in soil chemical properties, macro- and micronutrient concentrations in the soil surface (A_p1; 0–25 cm) and subsurface (A_p2; 25–50 cm) horizons, after CSS application with or without CMF in sugarcane cultivation (first and second ratoon cane). Eleven treatments, featured by CSS increase rates and mixed with CMF at different concentrations, were tested in the first ratoon; during the second, the CSS residual effect was evaluated. Applying CSS in sugarcane-cultivated soils, improved the following: (i) soil organic matter, pH, the sum of bases, cation-exchange capacity, and base saturation; (ii) overall nutrient concentrations (P, K, Ca, Mg, B, Cu, and Zn). The treatments showing the best performances were those with 5.0 Mg ha⁻¹ of CSS. Composted sewage sludge has the potential for use as an organic natural fertilizer reducing the need for CMF. When applied in infertile tropical soils, additional positive effects can be achieved, such as decreasing production costs and providing socio-economic benefits. Full article

In order to shorten the process of textile printing with natural dyes, develop new methods, and improve the color fastness and quality of printed products, this study presents a novel approach by synthesizing a natural complex dye through the interaction between purpurin and Fe²⁺ ions, resulting in a compound named purpurin–Fe²⁺ (P-Fe). This synthesized complex dye was meticulously characterized using state-of-the-art analytical techniques, including Fourier transform infrared spectroscopy (FT-IR), ultraviolet–visible (UV–Vis) spectrophotometry, and scanning electron microscopy energy-dispersive spectroscopy (EDS). The characterization confirmed the successful complexation of purpurin with Fe²⁺ ions. The prepared complex dye P-Fe was used for the printing of silk fabric. The optimized printing process involves steaming at a temperature of 100 °C for a duration of 20 min. In comparison to fabrics printed using direct dyes, the K/S values of the fabric printed with the P-Fe complex showed a significant enhancement, with all color fastness ratings achieving grade four. Furthermore, the proportion of metal elements on the white background of the printed fabric was found to be less than 0.180%, and the level of whiteness was above 50. The application of the P-Fe dye in silk fabric printing not only streamlines the printing process but also enhances the depth and speed of the printed color, effectively addressing the issue of color transfer onto a white background, which is commonly associated with natural dyes. Full article

Hyperlipidemia is the root cause of numerous chronic conditions, leading to high mortality rates around the globe. Spirulina (Arthrospira platensis) microalgae serve as a promising reservoir of bioactive compounds with diverse pharmacological properties. The current study examined the nutritional profile of spirulina powder in relation to strict glycemic control, specifically focusing on its potential to lower lipid levels. In an in vivo investigation, normal healthy male Wistar albino rats (n = 60) were divided into two groups: a negative control group (NC) of ten rats and a high-fat diet group (n = 50) that were fed a cholesterol-rich diet until their cholesterol levels reached or exceeded 250 mg/dL. Subsequently, the hypercholesterolemic rats were then randomly allocated to several treatment groups: a positive control (PC); a standard treatment diet (STD) involving fenofibrate at a dose of 20 mg/kg body weight; and three experimental groups (T1, T2, and T3) that received spirulina powder supplementation at doses of 300, 600, and 900 mg per kg body weight, respectively, for the period of 12 weeks. Blood samples were analyzed for oxidative stress biomarkers, insulin levels, lipid profiles, liver function, and expression of gene levels in the diabetogenic pathway. The study utilized spectrophotometric colorimetric methods to identify oxidative stress biomarkers, serum kit methods to measure lipid profiles and liver enzymes, and the assessment of qPCR for mRNA quantity. According to the research findings, spirulina powder has certain noteworthy features. It had the greatest quantity of chlorogenic acid (4052.90 µg/g) among seven phenolics and two flavonoid compounds obtained by HPLC-UV analysis. Furthermore, the proximate analysis demonstrated that spirulina is high in protein (16.45 ± 0.8%) and has a significant energy yield of 269.51 K-calories per 100 g. A maximal spirulina dose of 900 mg/kg/wt significantly lowered oxidative stress, cholesterol, triglyceride, low-density lipoproteins (LDL), and insulin levels (p ≤ 0.05). In contrast, high-density lipoprotein (HDL) and total antioxidant capacity (TAC) levels increased significantly (p ≤ 0.05) compared to all other groups, except the NC group. The study provides remarkable proof about the pharmacological impact of spirulina powders. Significant reductions (p ≤ 0.05) in liver enzymes {alanine aminotransferase (ALT) and aspartate aminotransferase (AST)} were observed across all treatment groups, with the exception of the NC, compared to the positive control. The treatment groups had significantly greater gene expression levels of INS-1, PDX-1, IGF-1, and GLUT-2 than the positive control group (p ≤ 0.05). These findings highlight spirulina’s potential as a long-term regulator of hyperglycemia in rat models with induced hyperlipidemia, owing to its phenolic bioactive components that serve as antioxidants. Full article

Lithium slag (LS), an industrial waste byproduct generated during lithium salt production, is characterized by its harmful trace elements, significant stockpiles and low pozzolanic activity. By 2003, the annual discharge of lithium slag in China surpassed 15 million tons, creating an urgent need for established large-scale disposal technologies. One of the primary strategies for the effective utilization of LS is its application as an auxiliary cementitious material in concrete. However, the low reactivity of LS and challenges associated with its large-scale application impede its effective utilization. Enhancing the pozzolanic activity of LS is pivotal for its substantial incorporation into concrete. This study begins by analyzing the physicochemical properties and volcanic ash reactivity of LS derived from various lithium extraction techniques. It subsequently explores the diverse activation techniques aimed at improving the reactivity of LS within concrete. Ultimately, this paper highlights the significance of synergistic activation strategies, particularly physicochemical co-excitation and multi-exciter composite excitation. These approaches are identified as critical pathways for enhancing the activity of LS. Through this exploration, this study aims to unveil innovative strategies that bolster the resource utilization efficiency of LS, thereby facilitating its effective application in the concrete domain. Full article

Assessing the new quality productive forces (NQPF) of new energy vehicle (NEV) companies is crucial for promoting the sustainable development of the NEV industry. This paper systematically evaluated and analyzed the NQPF of Chinese listed NEV companies from 2018 to 2022 using a novel multi-criteria decision analysis (MCDA) model. To address limitations in traditional MCDA models, such as unbalanced weight distribution, insufficient ranking differentiation, and incomplete identification of key influencing factors, this study introduced a new model, IDOCRIW-PROBID (integrated determination of objective criteria weights—preference ranking on the basis of ideal-average distance). First, an evaluation index system tailored to NEV companies’ NQPF was developed. Then, the IDOCRIW method was used to objectively assign weights to the indicators, enhancing the scientific rigor of the weight distribution. The PROBID method was employed to rank companies based on their NQPF, identifying differences between them. Additionally, an obstacle degree model was introduced to analyze key influencing factors, compensating for the traditional MCDA model’s limitations in this regard. The results showed, first, that the proposed IDOCRIW-PROBID model has a high degree of consistency with the classical Entropy-TOPSIS (technique for order of preference by similarity to ideal solution) model in terms of ranking the results (correlation coefficient = 0.91), and that IDOCRIW-PROBID offers higher differentiation compared to other MCDA models, validating its reliability and superiority. Second, during the study period, the development levels of NQPF in Chinese listed NEV companies varied significantly, with most companies at a low level of development and showing a downward trend, indicating that companies face considerable challenges in improving their NQPF. Third, the obstacle degree analysis revealed that R&D lease fees, R&D depreciation and amortization, and direct R&D investment were the primary factors hindering NQPF growth. This research provides theoretical support and decision-making insights for strategic optimization in NEV companies and informs government policy formulation. Full article

Efficient and clean treatment of wastewater and energy recovery and utilization are important links to realize low-carbon development of oilfields. Therefore, this paper innovatively proposes a multi-energy complementary co-production heating system which fully and efficiently utilizes solar energy resources, oilfield waste heat resources, and biomass resources. At the same time, a typical dormitory building in the oil region was selected as the research object, the system equipment selection was calculated according to the relevant design specifications. On this basis, the simulation system model is established, and the evaluation index and operation control strategy suitable for the system are proposed. The energy utilization rate of the system and the economic, energy-saving, and environmental benefits of the system are simulated. The results show that, under the simulated conditions of two typical days and a heating season, the main heat load of the system is borne by the sewage source heat pump, the energy efficiency is relatively low in the cold period, and the energy-saving characteristics are not obvious. With the increase in heating temperature and anaerobic reactor volume, the energy consumption of the system also increases, and the energy efficiency ratio of each subsystem and the comprehensive energy efficiency ratio of the system gradually decrease. In addition, although the initial investment in cogeneration heating systems is high, the operating costs and environmental benefits are huge. Under the condition of maintaining 35 °C, the anaerobic reactor in the system can reduce carbon emissions by 12.15 t per year, reduce sulfur dioxide emissions by 98.4 kg, reduce dust emissions by 49.2 kg, and treat up to 2700 t of sewage per year, which has broad application prospects. Full article

Utilizing long-wavelength laser diodes (LDs) for pumping to achieve high-power fiber laser output is an effective method for attaining high quantum efficiency and excellent thermal management. In this work, we report on a Master Oscillator Power Amplifier (MOPA)-structured long-tapered Yb³⁺-doped fiber laser directly pumped by long-wavelength laser diodes. By shifting the center wavelength of the pump source to 1010 nm, the heat generation within the fiber laser is effectively controlled, thereby increasing the transverse mode instability (TMI) threshold. Additionally, the use of a long-tapered fiber enlarges the mode area and suppresses stimulated Raman scattering (SRS) effects that typically arise from increased fiber length. As a result, an output of 6030 W is achieved with an optical-to-optical (O–O) efficiency of 83.7%, a SRS suppression ratio exceeding 50 dB, and no occurrence of dynamic TMI. This approach provides a valuable reference for optimizing long-wavelength pumping to suppress nonlinear effects and also holds potential for wide-temperature operational applications. Full article