Search Results (63,222)

The identity of community residents is the basis for passing on the GIAHS (globally important agricultural heritage system) and promoting the sustainable development of heritage sites. However, there is a lack of discussion in the existent literature on its composition and effective mechanisms. Based on the theory of the ABC (Affect–Behavior–Cognition) model, we collected 482 questionnaires from residents of tea communities of the GIAHS. We construct a model of multiple effects of identity on willingness to co-create values based on the perspective of host–guest interaction. The results show that identity has both social identity and self-identity dimensions. It has a valuable effect on the process of host–guest interaction. It stimulates residents’ willingness to co-create value for the GIAHS through the chain-mediating effect of welcoming nature and emotional closeness. The study results reveal the theoretical mechanisms by which the identity of residents influences the multiple behaviors of guests. It also provides an interactive perspective for the study of the participation of the community in the GIAHS. At the same time, it promotes the local practice of value mining and development research in the GIAHS. In addition, it expands the research framework of value co-creation in heritage tourism. Full article

Talent cultivation is the fundamental mission of higher education institutions, and the key to improving the quality of talent cultivation lies in enhancing the quality of teaching. In this regard, the Joint Committee recommends that the United Nations Educational, Scientific and Cultural Organization (UNESCO) should be invited to participate in this conference, in accordance with their respective mandates. However, in China, research on course evaluation systems and mechanisms in application-oriented universities is relatively scarce, and the evaluation dimensions are often limited; therefore, the evaluation of graduate courses in universities faces challenges such as a lack of specialized assessment systems, limitation of evaluation methods, and an imbalance between emphasis on outcomes and neglect of the teaching process. In this study, a comprehensive evaluation system for the Advanced Structural Dynamics (ASD) course is constructed based on the context-input-process-product (CIPP) evaluation model. The evaluation was conducted from four perspectives: teaching objectives, teaching inputs, teaching processes, and teaching outcomes. The fuzzy analytic hierarchy process (AHP) and simulated annealing particle swarm algorithm (SAPSO) are employed to study evaluation indicators and weights at various levels for the ASD course, and the proposed method is validated through practical examples. This study combines qualitative and quantitative evaluation indicators to achieve comprehensive assessment and adopts more scientifically rational algorithms for weight calculation, aiming to improve the accuracy and efficiency of weight calculation. The research findings of this study can further enhance the evaluation level of teaching quality and talent cultivation in graduate courses at application-oriented universities. Full article

Periprosthetic joint infections occur in 1–2% of all patients undergoing prosthetic joint surgeries. Although strong efforts have been made to reduce infection rates, conventional therapies like one- or two-stage revisions have failed to lower the infection rates. Cold atmospheric plasma (CAP) has shown promising results in reducing bacterial loads on surfaces. In this study, we aimed to investigate the ability of CAP to reduce the bacterial load on metal surfaces with varying distances and different plasma compositions below a temperature suitable for in vivo applications. Methods: Biofilm was formed with Staphylococcus aureus ATCC 29213 and Staphylococcus epidermidis ATCC 12228 cultures on TMZF discs. Plasma treatments using air plasma and argon plasma were conducted on discs containing the established biofilm while the temperature was measured. During the experiments, the duration and the distance of plasma application varied. Afterwards, colony-forming units were counted. Results: The results of this study showed that air and argon plasma could be considered for applications during surgeries at a 1 cm distance. While air plasma showed the highest efficiency in CFU reduction, the temperature generation due to the presence of oxygen poses a limitation concerning the duration of application. The use of argon as a plasma generator does not show the temperature limitation in correlation to exposure time. The use of air plasma with a distance of 1 cm to the application site and an exposure time of 5 s showed the most effective bacterial reduction while not exceeding tissue-damaging temperatures. Full article

Objective: Abnormally fertilized embryos are often discarded during in vitro fertilization due to the fact that known chromosomal ploidy abnormalities lead to implantation failure or pregnancy loss. The objective of this study was to determine if pronuclear numeration (PN) observed at fertilization check is representative of the true ploidy status of the subsequent developing blastocyst in order to maximize the number of viable embryos available for infertility patients and increase their chances of conception. Methods: Upon successful fertilization, pronuclear numeration was noted, and zygotes were cultured to the blastocyst stage. Biopsied trophectoderm cells were then lysed, and the isolated DNA was whole-genome amplified followed by library preparation. Next-generation sequencing was performed for PGT-A, and excess whole-genome amplified DNA was utilized for single nucleotide polymorphism beadchip array analysis. Results: At the time of fertilization check on day 1 of embryo development, when there were no visible pronuclei (n = 291), 56% of these 0PN blastocysts were confirmed to be diploid and normally fertilized. The remaining 41.9% were aneuploid, and 2.1% of the 0PN blastocysts contained only 23 haploid chromosomes. Upon analysis of the 1PN blastocysts (n = 217), just over a third (36.4%) only contained 23 haploid chromosomes (23XO), with another third (31.8%) identified as aneuploid, and surprisingly, the remaining third (31.8%) confirmed to be diploid and normally fertilized. In contrast, 50% of the 3PN blastocysts (n = 172) showed the presence of a third set of 23 parental chromosomes and were confirmed to be triploid (69XXY = 59.3% and 69XXX = 40.7%), with 41.9% identified as aneuploid and, interestingly, a small percentage (8.1%) confirmed to be diploid with normal fertilization. A very small proportion of biopsied blastocysts (0.63%) displaying the correct number of pronuclei for normal fertilization (2PN) were also identified as triploid with a third set of 23 parental chromosomes. To date, there have been 74 euploid embryo transfers from zygotes originally identified with an alternate pronuclear numeration, resulting in 16 ongoing pregnancies and 32 healthy live births, rates that match those typically observed with normally fertilized 2PN zygotes (>60%). Conclusions: A surprising number of blastocysts that were identified to have alternate pronuclear numeration at fertilization check on day 1 of embryo development were actually determined to be diploid with normal fertilization after molecular analysis. Accurate identification of haploid and tripoid zygotes is critical to prevent implantation failure and pregnancy loss and allows for the identification of all euploid embryos in a cohort, which has the potential to increase cumulative live birth rates for infertility patients. Full article

Penicillium expansum is a ubiquitous pathogenic fungus that causes blue mold decay of apple fruit postharvest, and another member of the genus, Penicillium chrysogenum, is a well-studied saprophyte valued for antibiotic and small molecule production. While these two fungi have been investigated individually, a recent discovery revealed that P. chrysogenum can block P. expansum-mediated decay of apple fruit. To shed light on this observation, we conducted a comparative genomic, transcriptomic, and metabolomic study of two P. chrysogenum (404 and 413) and two P. expansum (Pe21 and R19) isolates. Global transcriptional and metabolomic outputs were disparate between the species, nearly identical for P. chrysogenum isolates, and different between P. expansum isolates. Further, the two P. chrysogenum genomes revealed secondary metabolite gene clusters that varied widely from P. expansum. This included the absence of an intact patulin gene cluster in P. chrysogenum, which corroborates the metabolomic data regarding its inability to produce patulin. Additionally, a core subset of P. expansum virulence gene homologues were identified in P. chrysogenum and were similarly transcriptionally regulated in vitro. Molecules with varying biological activities, and phytohormone-like compounds were detected for the first time in P. expansum while antibiotics like penicillin G and other biologically active molecules were discovered in P. chrysogenum culture supernatants. Our findings provide a solid omics-based foundation of small molecule production in these two fungal species with implications in postharvest context and expand the current knowledge of the Penicillium-derived chemical repertoire for broader fundamental and practical applications. Full article

The highly valued oil of Rosa damascena Mill. (Rosaceae), widely used in high perfumery, cosmetics, and other spheres of human life, obliges us to know and study the safety profile of the product obtained from the water–steam distillation of fresh rose petals. The genotoxicity of the essential oil (EsO) has not been thoroughly studied despite its wide range of applications. That predetermined the object of this study—to evaluate, through classical cytogenetic methods, the possible cytotoxic/genotoxic activities of R. damascena Mill. EsO (EsORdm) in three different test systems: plant root meristem cells, mammalian bone marrow cells, and human lymphocyte cultures. The rose essential oil showed varying concentration- and time-dependent cytotoxic and genotoxic effects depending on the test system used, and it was established that the oil showed moderate cytotoxicity in lymphocyte cultures and non-high cytotoxicity in ICR mice but none in barley. Both barley and human lymphocytes showed a genotoxic effect with a dose-dependent increase in chromosomal aberrations (CAs) and a substantial rise in micronucleus (MN) frequency, while no genotoxicity was observed in bone marrow cells at the applied concentrations. Human lymphocytes exhibited the highest susceptibility to cytotoxic and genotoxic actions of the EsO. As a valuable plant-derived aromatic product with versatile uses in human life, R. damascena Mill. essential oil should be used in an appropriate concentration range tailored to cellular sensitivity. Full article

Background: Handball is characterized by fast and dynamic movements requiring appropriate psychomotor abilities and body mass composition. High levels of reaction and movement time can be crucial factors influencing quick reactions and in-time decision-making at the handball court. The aim of this study was to assess psychomotor abilities among elite and sub-elite Polish and Portuguese male and female handball players at the different levels of competition. Methods: Computer Test2Drive systems were used to assess reaction time, movement time and percentage of correct responses of 199 handball players (60 females). Results: Statistically significant correlation was noted between SIRT cr and the Elite group (r = 0.44) and between the CHORT cr and all groups (r = 0.33). A statistical correlation between CHORT MT and total body water (r = 0.44) was also noted in Elite handball players. Conclusions: High level of psychomotor abilities and body composition seems to have impact on the competitive level in male and female handball players. Full article

AI/AN communities are disproportionately impacted by food insecurity and gestational diabetes mellitus (GDM). Decreasing the risk of GDM can interrupt the intergenerational cycle of diabetes in AI/AN families, and can decrease diabetes-related health disparities. The goal of this study was to explore ways of supporting holistic health and reducing the risk of GDM among young American Indian and Alaska Native (AI/AN) females prior to pregnancy. Semi-structured interviews were conducted with adult AI/AN women (>18 years) who had GDM or who have young female relatives (e.g., daughters) (n = 41), AI/AN females between 12 and 24 years (n = 18), and key experts in food/nutrition and health within AI/AN communities (n = 32). Three themes emerged: (1) guidance on how to support young females’ holistic wellness; (2) evidence that generations of colonial violence, including forced removal, forced poverty, and the imposition of a Western-based food system, causes deeper, systemic fracturing of traditional cultural food knowledge and practices; and the fact that (3) opportunities for improved adolescent female health are rooted in AI/AN values and how AI/AN people resist the impacts of anti-Indigenous racism through family-based, community-led, and holistic health. These themes suggest alternative understandings about the relationships between food insecurity and holistic pre-pregnancy health and can guide our next steps in decreasing health disparities in these communities. Full article

This study investigated the impacts of light spectra on oxidative stress and nutrient quality of the fish Plectropomus leopardus in indoor recirculating aquaculture systems. The fish (100 g ± 0.45 g [wet weight]) were cultures in five different light spectra (full-spectrum (400‒800 nm), blue (450 nm), green (530 nm), red (630 nm), and dark) for 60 days. After experimentation, blood and muscle tissue were collected and analyzed for biochemical variables and nutritional quality. We demonstrated that the total cholesterol, triglycerides activities of P. leopardus in the dark groups were substantially elevated, relative to other groups (p < 0.05). Glutathione and glutathione peroxidase activities were elevated in the green light group versus other red groups, and cortisol was drastically reduced in the red group relative to other groups (p < 0.05). The crude ash concentration in the blue and full-spectrum group was substantially more elevated than in other groups (p < 0.05). Thr, Glu, Cys, Val, Met, Ile, Leu, Phe, Lys, His, Arg were markedly higher in the blue light versus the red light group (p < 0.05). The muscle of P. leopardus was rich in lysine and its essential AA index was in the order of blue light, full-spectrum, green light, dark and red group. The content of total saturated fatty acids in the blue light group was drastically lower relative to the dark, green and red groups (p < 0.05), and the total polyunsaturated fatty acids and DHA + EPA contents in the blue light group were substantially elevated relative to the other groups (p < 0.05). These results revealed that different light environments had certain effects on blood biochemical, antioxidant capacity, nutrient composition and proportion of P. leopardus. A comprehensive evaluation found that the blue light environment had more positive effects on the physiological, biochemical and nutritional quality of P. leopardus. This result provides a theoretical reference for the lighting strategy of an indoor recirculating aquaculture system. Full article

Imagine a future where language is no longer a barrier to real-time conversations, enabling instant and lifelike communication across the globe. As cultural boundaries blur, the demand for seamless multilingual communication has become a critical technological challenge. This paper addresses the lack of robust solutions for real-time face-to-face translation, particularly for low-resource languages, by introducing a comprehensive framework that not only translates language but also replicates voice nuances and synchronized facial expressions. Our research tackles the primary challenge of achieving accurate lip synchronization across culturally diverse languages, filling a significant gap in the literature by evaluating the generalizability of lip sync models beyond English. Specifically, we develop a novel evaluation framework combining quantitative lip sync error metrics and qualitative assessments by human observers. This framework is applied to assess two state-of-the-art lip sync models with different architectures for Turkish, Persian, and Arabic languages, using a newly collected dataset. Based on these findings, we propose and implement a modular system that integrates language-agnostic lip sync models with neural networks to deliver a fully functional face-to-face translation experience. Inference Time Analysis shows this system achieves highly realistic, face-translated talking heads in real time, with a throughput as low as 0.381 s. This transformative framework is primed for deployment in immersive environments such as VR/AR, Metaverse ecosystems, and advanced video conferencing platforms. It offers substantial benefits to developers and businesses aiming to build next-generation multilingual communication systems for diverse applications. While this work focuses on three languages, its modular design allows scalability to additional languages. However, further testing in broader linguistic and cultural contexts is required to confirm its universal applicability, paving the way for a more interconnected and inclusive world where language ceases to hinder human connection. Full article

This article explores recent advancements in non-destructive spectroscopic techniques for identifying pigments in polychrome cultural relics, emphasizing their significance for sustainability in cultural heritage preservation. Techniques such as infrared spectroscopy, Raman spectroscopy, and X-ray fluorescence spectroscopy facilitate the accurate identification of pigment compositions while ensuring the integrity of the artifacts is maintained. Our findings indicate that integrating multiple spectroscopic methods enhances the accuracy of pigment identification and deepens our understanding of the structural and preservation status of historical artifacts. We also outline future directions for spectroscopic analysis in the field of cultural heritage, including micro-area analysis, data fusion, and intelligent data processing, aimed at improving the efficiency and effectiveness of pigment identification, ultimately contributing to the sustainable preservation of cultural assets. Full article

Paper-based artworks are prone to natural aging processes driven by chemical and biological processes. Numerous treatments have been developed to mitigate deterioration and prevent irreversible damage. In this study, we investigated the use of poly(acrylic acid)/TiO₂ composite hydrogels, combining their cleaning and protective functions in a minimally invasive treatment. Hydrogels allow for controlled water flow and photocatalytic TiO₂ nanoparticles enhance the hydrogel’s efficacy by enabling the removal of oxidation products and inactivating biological contaminants. Furthermore, this innovative material can act as a protective coating against UV-induced aging, preserving both color and stability of the paper. Raman spectroscopy and confocal laser scanning microscopy imaging techniques were employed to evaluate the treatments, allowing for us to differentiate between hydrolytic and oxidative aging processes. Our findings demonstrate that papers coated with poly(acrylic acid)/TiO₂ composite hydrogels exhibit significant reductions in oxidative markers, an enhanced color stability, and an overall improved resistance to degradation compared to uncoated samples. Full article

The assessment of the various skills of athletes is carried out in terms of their ability to perform sport-specific tasks. The cognitive abilities of the players have significance for their effectiveness. In volleyball, a player’s ability to react quickly appears to be crucial in responding to an opponent’s dynamic play. The aim of the study was to evaluate the changes in reaction time to light signals following warm-up and physical exercise. Eighteen volleyball players (15.58 ± 2.01 years) participated in the study. Four FITLIGHT Trainer^TM discs were placed on the wall facing the participant to conduct the reaction time test. The participant’s task was to react as quickly as possible to the illuminated disc by touching it with the palm of their hand. The procedure was repeated five times: before the warm-up, after the warm-up, and after each of the three agility tests. Friedman’s ANOVA showed statistically significant differences in reaction time between the tests (Chi² ANOVA = 61.23; p < 0.001). All tests performed after the warm-up according to the RAMP protocol showed statistically significantly better results than those before the warm-up (p ≤ 0.05). At the same time, no differences were observed between the tests performed after successive agility tests. The results indicated that a well-chosen warm-up plays an important role in shortening the time of visual-motor reaction to a light stimulus (RT). Subsequent studies should be expanded to include other research groups and assess other parameters. Full article

Although vinegar is a product obtained by a well-known bioprocess from a technical point of view, the complex microbiota responsible for its production and their involvement in the organoleptic profiles are not clear yet. In this work, three acetification profiles in submerged culture using both synthetic and raw materials from Andalusia (Spain) were characterized by metagenomic (16S rRNA amplicon sequencing) and metabolomic tools (stir-bar sorptive extraction with thermo-desorption coupled to gas chromatography–mass spectrometry (SBSE-TD-GC−MS) and high-performance liquid chromatography (HPLC)). A total of 29 phyla, 208 families, and many more genera were identified, comprising bacteria and archaea as well as 75 metabolites, including minor volatile compounds, amino acids, biogenic amines, and other nitrogenous compounds. It can be concluded that Komagataeibacter and Acetobacter were not only the predominant genera but also the ones that most influenced vinegar metabolite profiles by using different metabolic strategies for mutual collaboration, and together with other microbial groups, some of them were previously practically unknown in vinegar. These results can be of interest not only to deepen the basic knowledge about vinegar but also to the vinegar industry by elucidating microbial succession and the key associated metabolites. Full article

This research explores the perceptions of tourists regarding the state and sustainable management of tangible cultural heritage in the city of Porto (Portugal), the destination recognised for its historical and cultural heritage. Porto attracts a growing number of tourists due to its rich heritage, encompassing cultural, architectural, and scenic elements. This rising demand necessitates sustainable management practices to protect the city’s heritage and ensure long-term sustainability. With the increase in tourism and the need to preserve cultural and environmental resources, sustainable management becomes essential to balance economic development and heritage conservation. The study’s main objective is to analyse tourists’ perspectives on the sustainable management of the cultural heritage of the city of Porto. It focuses on analysing current tourism management practices and calls for new strategies to promote sustainability. Structured questionnaires were distributed to a random sample of tourists in strategic locations between January and April 2023, obtaining quantitative data. The sample consists of 264 respondents. The results revealed four main factors, showing that most respondents show a high level of awareness and appreciation of sustainability and its relevance for heritage conservation. Regarding tourism, the majority agree that it contributes positively to the conservation and restoration of the city’s heritage. Full article