Search Results (4,347)

Skin aging is accelerated by inflammation processes generated by oxidative stress and external factors such as UV radiation. Plants belonging to the genus Kalanchoe that are rich sources of antioxidants could potentially strengthen the skin barrier if used as ingredients in cosmetic formulations. However, their use is limited due to the contents of bufadienolides, known cardiotoxins. This study aimed to establish a semi-quantitative profile of bufadienolides in the juices of K. blossfeldiana, K. daigremontiana, and K. pinnata using UHPLC combined with charged aerosol detection (CAD) and high-resolution mass spectrometry (HR-MS). Additionally, the study determined the ability of bufadienolides to penetrate the skin barrier using the Bronaugh Diffusion Cell Apparatus and Strat-M membrane. The study also assessed the ferric and molybdenum-reducing powers, as well as the radical scavenging capabilities of these plants juices using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) methods. The in vitro antihyaluronidase and antityrosinase activities and sun protection factor (SPF) were evaluated spectrophotometrically, indicating moderate capability to inhibit the skin enzymes, but low SPF protection for all analyzed juices. The semi-qualitative analysis demonstrated the presence of bufadienolides occurring in two juices from K. daigremontiana and K. pinnata, with the highest contents of 1,3,5-bersaldegenin-orthoacetate, bryophyllin-A/bryotoxin-C, bersaldegenin-acetate/bryophyllin-C, and diagremontianin. After passing through the skin model, no bufadienolide compounds were present in the subcutaneous filtrate. Antiradical and reduction assays revealed the antioxidant potential of K. blossfeldiana and K. pinnata. These results indicate that Kalanchoe juices have antiaging potential and appear safe for dermal applications. Full article

Background: Replacing a maxillary central incisor and immediately placing an implant represents a clinical challenge. Methods: This case report demonstrates a full digital workflow to achieve a predictable implant placement and esthetically pleasing restoration for a 35-year-old male patient who suffered a horizontal root fracture after a sports accident. The patient’s treatment included digital implant planning, minimally traumatic tooth extraction, computer-guided implant placement, soft tissue augmentation, and a provisional restoration to contour the augmented gingival tissues. The process began with cone beam computed tomography (CBCT) evaluation and patient consultation on treatment options, with the patient opting for implant therapy. Using a 3D-printed surgical guide, the implant was placed precisely, and a soft tissue graft was used to enhance the gingival architecture and volume. A provisional restoration was designed to support the emergence profile and condition the peri implant soft tissues. A final digital impression was made, and a screw-retained all-ceramic crown was fabricated uneventfully after healing. Results: This digital approach allowed accurate planning and allowed the efficient execution of a technique-sensitive procedure such as immediate implant placement, thus providing an esthetic and functional solution while minimizing treatment time. Conclusions: The case highlights that immediate implant therapy in the esthetic zone requires meticulous planning and execution, and that incorporating advanced digital tools and techniques is required to achieve favorable clinical outcomes. Full article

The 7.7 and 7.6 magnitude Pazarcık and Elbistan earthquakes that struck Kahramanmaraş on 6 February 2023 caused widespread structural damage across many provinces and are considered rare in seismological terms. While many reinforced concrete (RC) buildings designed under current earthquake regulations sustained significant damage, some older RC buildings with outdated designs sustained only moderate damage. This study aims to analyze the seismic performance of such older RC buildings to understand why they did not collapse or suffer severe damage. An 8-story RC building in Adıyaman province, damaged by the earthquake, was considered for analysis. The region’s seismicity and local site conditions were assessed through borehole operations, geotechnical laboratory tests, and seismic field tests. The soil profile was modeled, and one-dimensional seismic site response analyses were performed using records from nearby stations (TK 4615 Pazarcık and TK 4612 Göksun stations) to determine the foundation-level earthquake record. Nonlinear static pushover analysis was carried out via SAP2000 and STA4CAD, utilizing site response analysis and test results taken from the reinforcement and concrete samples of the building. The findings, compared with the observed damage, provide insights into the performance of older RC buildings in this region. Full article

Cultural heritage is a common good passed down as a legacy from previous to future generations. Its preservation is a strong commitment to humanity. The main motivation for this project is based on this understanding and arose from the need for the proper and scientifically documented recording of cultural heritage (CH), both movable and immovable monuments of ecclesiastical cultural treasures. Despite its significance, the systematic documentation of ecclesiastical heritage remains fragmented, lacking a standardized and scientifically driven approach. This research addresses this critical gap by developing a structured methodology for the recording, organization, and digital archiving of ecclesiastical CH monuments. This was accomplished by codifying the actual recording and documentation needs for the ecclesiastical cultural treasures, with the systematic study of the users’ needs. The study focused on the region of Thrace, encompassing areas of Greece, Turkey, and Bulgaria, where post-Byzantine ecclesiastical treasures are expected to be in abundance. Through the design and implementation of surveys and metadata collection, this project has the capacity to facilitate digital transformation across the interconnected fields of religion, arts, and CH. Stakeholders from diverse backgrounds, both within and outside the clergy community, including owners and end-users connected to ecclesiastical cultural treasures, were actively involved in the process. The COVID-19 pandemic underscored the vital role of building distance communication channels and promoting digital transformation across the interconnected fields of religion and cultural heritage. Our emphasis was to actively engage stakeholders from diverse backgrounds to create a practical, user-friendly documentation tool that meets their actual needs. Full article

This paper presents an integrated CAD/CAM system that can be used for Computer Aided Process Planning to interact or automate 3D/4D/5D printing process towards Industry 5.0. By creating a new algorithm, macro is developed that has the capability to determine any suitable type of printing process whether 3D, 4D or 5D printing for generating Additive Manufacturing products. The methodology of this study is focused starting from designing 3D CAD models for Additive Manufacturing products until generating 3D/4D/5D printing process planning by a creation of macro. The algorithm is tested by printing simulations from 3D printing extended up to 5D printing. Full article

This paper examines the digital transformation of the textile and fashion industry, focusing on the alignment with sustainability principles through the integration of Industry 4.0 technologies. The introduction highlights the urgency of transitioning from conventional production methods to innovative, digitally enabled systems that promote a circular economy and resource efficiency. The main research questions address the contribution of Industry 4.0 elements to sustainable solutions, the directions of digitalization within the apparel sector, and the significant impact of digital technologies on the achievement of sustainability goals. The theoretical framework examines sustainability in the textile industry and emphasizes the need for a green transformation facilitated by digital technologies to reduce environmental impacts. Industry 4.0 concepts, as discussed in The Concept of Industry 4.0 in the Textile and Apparel Sector, are revolutionizing production through technologies such as IoT, AI, and blockchain, enabling traceability, customization, and energy-efficient operations. The paper also explores the evolution of the fashion and apparel industry into a high-tech sector, highlighting advances such as CAD-CAM systems, digital printing, and 3D technologies that improve precision, reduce waste, and support sustainable practices. In its conclusion, the paper emphasizes the crucial role of interdisciplinary collaboration, regulatory frameworks, and investment in skills development to overcome the challenges of implementing digital and sustainable practices. It posits that a strategic embrace of digital ecosystems and Industry 4.0 technologies is essential for creating a resilient and sustainable textile industry that is aligned with environmental and societal goals. Full article

People with HIV (PWH) have a high risk of coronary artery disease (CAD). Cytomegalovirus (CMV) co-infection is very common in PWH, but little is known about its association with CAD. We aimed to investigate if CMV IgG serostatus and concentrations are associated with CAD in PWH. From the Copenhagen Comorbidity in HIV Infection (COCOMO) Study we included PWH with coronary CT angiography (CCTA) and quantitative CMV IgG concentration measurements. We measured the stenosis grades and plaque volumes in the coronary vessels. Using multivariable regressions adjusted for traditional CAD risk factors, we investigated if CMV IgG serostatus and concentrations were associated with any atherosclerosis, obstructive CAD, or plaque volumes. We included 620 PWH of whom 586 had positive CMV serostatus, which was not associated with any atherosclerosis, obstructive CAD, or plaque volumes. A doubling of CMV IgG concentrations was associated with any atherosclerosis (OR 1.21 [95% CI: 1.06–1.39]), obstructive CAD (OR 1.31 [95% CI: 1.07–1.59]), and higher total plaque volume (1.56 [95% CI: 1.21–2.01] fold increase), but the association did not remain significant after adjustment for traditional CAD risk factors. This indicates that CMV IgG serostatus and concentrations are not independently associated with prevalent CAD in PWH. Full article

Background and Clinical Significance: Renal abscess represents one infectious urological complication with lethal potential. The treatment of this pathology may differ depending on the severity of the symptoms and the size of the infectious collection. Diabetes, immunosuppression, and associated urinary pathologies are most frequently responsible for the development of abscesses. This case report presents the first documented case of a renal abscess associated with Sodium-glucose cotransporter 2 (SGLT2) inhibitors in a person without previous predisposing pathologies. Case Presentation: A 62-year-old patient presented to the emergency department for pain in the right flank, vomiting, and dysuria. The patient’s medical history revealed Heart Failure New York Heart Association (NYHA) Class II, Coronary Artery Disease (CAD) with prior angioplasty, and permanent Atrial Fibrillation. No prior urological or immunosuppressive conditions were detected. The Computed Tomography (CT) evaluation confirmed the ultrasound suspicion of a right renal abscess performed in the emergency room. The only risk factor identified was the initiation of SGLT2 inhibitor therapy for cardiac pathology approximately 2 months before. According to the small dimensions and urine culture, the abscess was successfully treated with antibiotic administration in collaboration with the urology department. The infectious process was remitted within 2 weeks. Conclusions: To our knowledge this is the first documented case of a renal abscess associated with SGLT2 inhibitor administration in a person without previous predisposing risk factors. Despite the relatively low incidence of urinary tract infections (UTIs) associated with SGLT2 inhibitors, their widespread use in the treatment of various socially significant conditions highlights the need for both patients and medical specialists to be aware of all potential risks and pay increased attention to these cases. Full article

Coronary artery disease (CAD) is linked to atherosclerosis plaque formation. In pro-inflammatory conditions, human Natural Killer (NK) cell frequencies in blood or plaque decrease; however, NK cells are underexplored in CAD pathogenesis, inflammatory mechanisms, and CAD comorbidities, such as human cytomegalovirus (HCMV) infection and diabetes. Analysis of PBMC CITE-seq data from sixty-one CAD patients revealed higher blood NK cell SPON2 expression in CAD patients with higher stenosis severity. Conversely, NK cell SPON2 expression was lower in pro-inflammatory atherosclerosis plaque tissue with an enriched adaptive NK cell gene signature. In CAD patients with higher stenosis severity, peripheral blood NK cell SPON2 expression was lower in patients with high HCMV-induced adaptive NK cell frequencies and corresponded to lower PBMC TGFβ transcript expression with dependency on diabetes status. These results suggest that high NK cell SPON2 expression is linked to atherosclerosis pro-homeostatic status and may have diagnostic and prognostic implications in cardiovascular disease. Full article

The prevalence of kidney stones, a significant urological health concern, necessitates advancements in the management and treatment methods, particularly in the domain of ureteral stents. This study explores the feasibility and potential benefits of utilizing three biodegradable polymers—Polylactic Acid (PLA), Tough Polylactic Acid (Tough PLA), and Polylactic Acid/Poly-hydroxybutyrate (PLA/PHB)—for the fabrication of 3D-printed ureteral stents tailored to patient-specific needs. Through the integration of CAD and Fused Deposition Modeling (FDM) 3D printing technology, ureteral stents were successfully produced, demonstrating key advantages in terms of biodegradability and mechanical properties. The study involved a rigorous evaluation of the biodegradability, tensile strength, and hardness of the stents. Biodegradability tests performed in a simulated physiological environment revealed that PLA/PHB and Tough PLA stents exhibited higher degradation rates compared to PLA, aligning with the requirements for temporary urinary tract support. Tensile strength testing indicated that while PLA showed the highest strength, PLA/PHB and Tough PLA stents provided beneficial ductility, reducing the risk of blockage due to material breakage. Hardness assessments classified PLA/PHB stents as medium soft, optimizing patient comfort during the stenting period. These findings demonstrate the potential of using biodegradable polymers to produce ureteral stents that could eliminate the need for removal procedures, thereby enhancing patient recovery and comfort. Full article

This study presents an innovative 4D digital model that integrates Bridge Information Modeling (BrIM) with several types of data to defect detection in complex bridge structures. The model promotes precise data preparation, navigation, visualization, integration, and monitoring, enabling the identification of defects, like material deterioration, condition changes, and structural clashes in components like trusses. Bridge model provides time-based access to maps, allowing users to explore changes over time and predict future conditions. The integration of time dimension into the 4D model provides dynamic tools for exploring changes over time, allowing for analysis and maintenance planning. Through the use of advanced 4D simulation technology, the study’s effectiveness is in visualizing workflows, identifying constraints, and supporting proactive decision-making in structural management. By incorporating various perspectives and enabling users to interact with detailed visualizations, the model enhances understanding and maintenance practices. This approach advances defect modeling and digitization, supporting automation in defect detection while significantly contributing to the long-term safety and sustainability of bridges. In order to obtain non-destructive images and films of the morphology of the sandstone’s internal structure at the bridge pier in addition to the stone’s grain texture and surface characteristics, this research applied X-ray computed tomography approach (CT scan) and XRF as NDT to the analysis of sandstone. Full article

Dihydroorotase (DHOase; EC 3.5.2.3) is a zinc-dependent metalloenzyme that plays a key role in the de novo pyrimidine biosynthesis pathway, catalyzing the reversible cyclization of N-carbamoyl aspartate to dihydroorotate. This reaction is essential for the production of uridine monophosphate, the precursor of all pyrimidine nucleotides required for DNA and RNA synthesis. Despite its conserved enzymatic function, DHOase exhibits significant structural diversity across species, particularly in its oligomeric states, gene fusion patterns, and active site architecture. A crucial structural feature of DHOase is its flexible active site loop, which undergoes dynamic conformational changes during catalysis. Previously, the loop-in conformation was associated with substrate binding, whereas the loop-out conformation was linked to product release and non-substrate ligand binding. However, recent crystallographic studies challenge this paradigm, revealing that certain non-substrate ligands and inhibitors, including malate, 5-fluoroorotate, plumbagin, 5-aminouracil, and 5-fluorouracil, interact with DHOase via a loop-in binding mechanism rather than the previously assumed loop-out mode. These findings necessitate a reassessment of the catalytic mechanism of DHOase and underscore the active site loop as a potential target for drug development. This review revisits the structural and biochemical mechanisms of DHOase, with a focus on recent crystallographic insights that redefine the loop-in binding mode for ligand interaction. By leveraging the unique conformational dynamics of the active site loop, novel inhibitors may be developed to selectively target pyrimidine biosynthesis in cancer cells and microbial pathogens. These insights emphasize the crucial role of structural biology in therapeutic design and highlight DHOase as a promising drug target. Full article

Vascular endothelial function plays an important role in the pathogenesis of atherosclerosis. The reduction in low-density lipoprotein cholesterol (LDL-C) is a key therapy for preventing coronary artery disease (CAD), but the role of omega-3 fatty acids as residual risk factors of CAD remains controversial. We studied the correlation between serum omega-3 fatty acid levels and endothelial function in patients with CAD receiving statin therapy and examined the effect of eicosapentaenoic acid (EPA) therapy on endothelial function. Methods: A total of 150 consecutive patients with CAD receiving statin therapy (LDL-C levels < 100 mg/dL) were enrolled. Serum omega-3 fatty acid levels were measured, and endothelial function was assessed by flow-mediated dilation (FMD) of the brachial artery. Subsequently, 65 patients with impaired FMD (<6%) and low EPA/arachidonic acid (AA) (<0.3) were administered EPA, and FMD was reassessed after 3 months. Results: A multivariate linear regression analysis demonstrated that serum docosahexaenoic acid (DHA) and EPA plus DHA levels were independent determinants of %FMD (β = 0.214 and 0.163, p < 0.05, respectively). The EPA therapy significantly improved %FMD (from 3.7 ± 1.0% to 4.1 ± 1.0%, p < 0.05) in patients with low EPA/AA, and especially in patients with low EPA/AA and high triglyceride levels (from 3.4 ± 1.0% to 4.0 ± 1.1%, p < 0.01). Conclusions: Serum omega-3 fatty acid levels were associated with endothelial dysfunction in patients with CAD receiving statin therapy. EPA therapy improves endothelial function in patients with low EPA/AA, especially those with low EPA/AA and high triglycerides. Full article

Background and Objectives: The manufacturing of single crowns using hybrid abutments is an alternative that may be interesting in clinical practice, combining the advantages of the different materials used in a personalized design for each case. The purpose of this in vitro study was to evaluate the torque loss, survival, reliability, failure mode, and strain distribution of implant-supported prostheses with zirconia (Zir) and cobalt–chromium (Co-Cr) hybrid abutments. Materials and Methods: Abutments were milled by CAD/CAM and divided into two groups according to the materials used, Zir and Co-Cr, and cemented on titanium bases screwed to dental implants. Monolithic zirconia crowns were cemented on the abutments. The implant/abutment/crown sets were subjected to thermomechanical cycling (n = 10) (2 Hz, 140 N, 1 × 10⁶ cycles, immersed in water at 5–55 °C) to evaluate the torque loss. The single load to fracture test (SLF) was performed to design the loading profiles (light, moderate, and aggressive) of the step-stress accelerated life testing (SSALT) (n = 21) to evaluate the survival and reliability. The representative fractured specimens were analyzed under optical and scanning electron microscopy. The digital image correlation (DIC) (n = 1) was performed using specimens embedded in polyurethane resin models that received static point loading, and the strain distribution was analyzed. Results: There was no difference in torque loss, survival, or reliability between zirconia and Co-Cr abutments. An analysis of the fractured surfaces showed that the abutments presented the same failure mode, where the fracture probably started in the titanium base/screw. The zirconia abutment model presented only compressive strains around the implant, while the Co-Cr abutment model showed tensile and compressive strains in the middle of the implant; however, all strains were within the clinically acceptable limits. There was a strain concentration in the titanium base close to the implant platform for both groups. Conclusions: Zirconia and Co-Cr hybrid abutments presented similar torque loss, survival, reliability, and failure modes, but the abutment material influenced the strain distribution around the implant. The titanium base screw was the weakest link in the system. Full article

This study presents a novel approach to the dehydration of n-propanol using three hybrid methods—D + HPV, D + HPV + D, and D + HPV + D with heat integration—each combining distillation (D) and hydrophilic pervaporation (HPV) without the use of additional solvent agents, as in the most common separation method, extractive distillation. The optimization was performed using a ChemCAD process simulator, targeting 99.9 wt% purity for n-propanol and water. This is the first research to provide a comprehensive cost estimation and carbon footprint analysis for such configurations. Results show the D + HPV + D + HI method provides the best balance of energy efficiency, environmental sustainability, and economic feasibility. It reduced heat duties by 18.5% compared to D + HPV + D, achieved similar CO₂ emissions to D + HPV with better energy efficiency, and lowered the total annual cost by 37.9% compared to D + HPV. The findings establish D + HPV + D + HI as a promising technology for sustainable and cost-effective n-propanol dehydration. Full article