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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Cancer Res. 2020 Jan 3;80(5):964–975. doi: 10.1158/0008-5472.CAN-19-1532

Figure 1.

Figure 1.

RAB11B-AS1 is induced by hypoxia in breast cancer cells in a HIF-2-dependent manner. A, RAB11B-AS1 RNA levels in breast cancer cell lines exposed to 20% or 1% O2 for 24 hours. MDA231, MDA-MB-231; MDA468, MDA-MB-468. B, 5’ and 3’ RACE amplicons of RAB11B-AS1 using total RNA isolated from MDA-MB-231 exposed to 1% O2 for 24 hours as a template. The location of 5’ and 3’ RACE PCR amplicons at the RAB11B-AS1 gene is illustrated on the top. bp, base pair. E, exon. C-F, RAB11B-AS1 RNA levels and HIF protein levels in parental, HIF-1α KO (C, D), HIF-2α KO (C, D), HIF-1/2α DKO (C, D), and HIF-1β KO (E, F) MDA-MB-231 cells exposed to 20% or 1% O2 for 24 hours (mean ± SEM, n = 3). **p < 0.01, ***p < 0.001, ****p < 0.0001.