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. 1971 Jun;68(6):1102–1106. doi: 10.1073/pnas.68.6.1102

A Unique Synaptosomal Fraction, Which Accumulates Glutamic and Aspartic Acids, in Brain Tissue

Alan R Wofsey 1,2,3, Michael J Kuhar 1,2,3, Solomon H Snyder 1,2,3,*
PMCID: PMC389128  PMID: 4331081

Abstract

Subcellular fractionation of rat cerebral cortical slices on sucrose density gradients provides evidence for the existence of a unique synaptosomal fraction (enriched in pinched-off nerve endings) that selectively accumulates glutamic and aspartic acids. The particles in this fraction sediment to a less dense portion of sucrose gradients than do particles that accumulate aromatic, basic, and neutral (large and small) amino acids. Particles that store gamma-aminobutyric acid are even less dense than those that contain exogenous glutamic and aspartic acids. The distribution of endogenous glutamic acid encompasses both that of exogenous glutamic acid and that of the neutral and basic amino acids. These findings provide neurochemical support for the suggestion that glutamic and/or aspartic acid has a specialized synaptic function, perhaps as a neurotransmitter, in the mammalian brain.

Keywords: rat, rabbit, neurotransmitters, sucrose density gradients

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blackburn K. J., French P. C., Merrills R. J. 5-hydroxytryptamine uptake by rat brain in vitro. Life Sci. 1967 Aug 1;6(15):1653–1663. doi: 10.1016/0024-3205(67)90176-2. [DOI] [PubMed] [Google Scholar]
  2. Blasberg R. G. Specificity of cerebral amino acid transport: a kinetic analysis. Prog Brain Res. 1968;29:245–258. doi: 10.1016/S0079-6123(08)64160-6. [DOI] [PubMed] [Google Scholar]
  3. CURTIS D. R., PHILLIS J. W., WATKINS J. C. The chemical excitation of spinal neurones by certain acidic amino acids. J Physiol. 1960 Mar;150:656–682. doi: 10.1113/jphysiol.1960.sp006410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Robertis E. Ultrastructure and cytochemistry of the synaptic region. The macromolecular components involved in nerve transmission are being studied. Science. 1967 May 19;156(3777):907–914. doi: 10.1126/science.156.3777.907. [DOI] [PubMed] [Google Scholar]
  5. Gfeller E., Kuhar M. J., Snyder S. H. Neurotransmitter-specific synaptosomes in rat corpus striatum: morphological variations. Proc Natl Acad Sci U S A. 1971 Jan;68(1):155–159. doi: 10.1073/pnas.68.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glowinski J., Iversen L. L. Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain. J Neurochem. 1966 Aug;13(8):655–669. doi: 10.1111/j.1471-4159.1966.tb09873.x. [DOI] [PubMed] [Google Scholar]
  7. Graham L. T., Jr, Aprison M. H. Fluorometric determination of aspartate, glutamate, and gamma-aminobutyrate in nerve tissue using enzymic methods. Anal Biochem. 1966 Jun;15(3):487–497. doi: 10.1016/0003-2697(66)90110-2. [DOI] [PubMed] [Google Scholar]
  8. Green A. I., Snyder S. H., Iversen L. L. Separation of catecholamine-storing synaptosomes in different regions of rat brain. J Pharmacol Exp Ther. 1969 Aug;168(2):264–271. [PubMed] [Google Scholar]
  9. Iversen L. L., Neal M. J. The uptake of [3H]GABA by slices of rat cerebral cortex. J Neurochem. 1968 Oct;15(10):1141–1149. doi: 10.1111/j.1471-4159.1968.tb06831.x. [DOI] [PubMed] [Google Scholar]
  10. KRNJEVIC K., PHILLIS J. W. Iontophoretic studies of neurones in the mammalian cerebral cortex. J Physiol. 1963 Feb;165:274–304. doi: 10.1113/jphysiol.1963.sp007057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuhar M. J., Green A. I., Snyder S. H., Gfeller E. Separation of synaptosomes storing catecholamines and gamma-aminobutyric acid in rat corpus striatum. Brain Res. 1970 Jul 29;21(3):405–417. doi: 10.1016/0006-8993(70)90420-8. [DOI] [PubMed] [Google Scholar]
  12. Kuhar M. J., Shaskan E. G., Snyder S. H. The subcellular distribution of endogenous and exogenous serotonin in brain tissue: comparison of synaptosomes storing serotonin, norepinephrine, and gamma-aminobutyric acid. J Neurochem. 1971 Mar;18(3):333–343. doi: 10.1111/j.1471-4159.1971.tb11962.x. [DOI] [PubMed] [Google Scholar]
  13. Kuhar M. J., Snyder S. H. The subcellular distribution of free H3-glutamic acid in rat cerebral cortical slices. J Pharmacol Exp Ther. 1970 Jan;171(1):141–152. [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Roberts E., Kuriyama K. Biochemical-physiological correlations in studies of the gamma-aminobutyric acid system. Brain Res. 1968 Apr;8(1):1–35. doi: 10.1016/0006-8993(68)90170-4. [DOI] [PubMed] [Google Scholar]
  16. Roberts E., Kuriyama K. Biochemical-physiological correlations in studies of the gamma-aminobutyric acid system. Brain Res. 1968 Apr;8(1):1–35. doi: 10.1016/0006-8993(68)90170-4. [DOI] [PubMed] [Google Scholar]
  17. Ross S. B., Renyi A. L. Inhibition of the uptake of tritiated 5-hydroxytryptamine in brain tissue. Eur J Pharmacol. 1969 Sep;7(3):270–277. doi: 10.1016/0014-2999(69)90091-0. [DOI] [PubMed] [Google Scholar]
  18. Shaskan E. G., Snyder S. H. Kinetics of serotonin accumulation into slices from rat brain: relationship to catecholamine uptake. J Pharmacol Exp Ther. 1970 Nov;175(2):404–418. [PubMed] [Google Scholar]
  19. Snyder S. H., Green A. I., Hendley E. D. Kinetics of H3-norepinephrine accumulation into slices from different regions of the rat brain. J Pharmacol Exp Ther. 1968 Nov;164(1):90–102. [PubMed] [Google Scholar]
  20. Snyder S. H., Green A., Hendley E. D., Gfeller E. Noradrenaline: kinetics of accumulation into slices from different regions of rat brain. Nature. 1968 Apr 13;218(5137):174–176. doi: 10.1038/218174a0. [DOI] [PubMed] [Google Scholar]

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