Figure 3.
Upregulation of inflammatory genes in the NYUHJD Learning Cohort and NYUHJD and Duke Validation Cohorts. A, Real Time PCR analysis: relative expression levels of inflammatory gene (IL-1β, TNFα, IL-8, IL-6, COX-1 and COX-2) expression in OA PBMCs (NYUHJD Learning Cohort). The raw QPCR data were normalized against housekeeping gene GAPDH, and relative expression levels were calculated using the delta-delta CT method (22). These data show that five of these genes are differentially over-expressed in the OAIL-1 group as compared to non-OA controls (NC) and OAnl. B and C, distributions of IL-1β and TNFα expression levels by group in two validation cohorts. Real Time PCR analysis: relative expression levels of IL-1β in (B) the NYUHJD Validation Ccohort and (C) Duke Validation Cohort by group. These data show that the IL-1β gene is differentially overexpressed in the OAIL-1group as compared to non-OA controls (NC) and OAnl. Box-Cox transformations were applied to normalize variables prior to regression modeling.