Abstract
A plasmid of 45 kb, designated pNOB8, was found in high copy number in a new heterotrophic Sulfolobus isolate, NOB8H2, from Japan. Dissemination of the plasmid occurred in six cultures of nine different Sulfolobus strains when small amounts of the donor were added. These mixed cultures exhibited a high average copy number of the plasmid, between 20 and 40 per chromosome, and showed a marked growth retardation. Horizontal transfer of pNOB8 was proved by isolating transcipients from mating mixtures via single colonies. In these isolates, the copy number of the plasmid appeared to be subject to a control mechanism. Cell-free filtrates of donor cultures did not transmit the plasmid, and plating of the donor on lawns of recipients did not result in plaque formation, suggesting that the transfer was not mediated by a virus. Rapid formation of cell-to-cell contacts between differently stained donor and recipient partners was demonstrated after the two strains were mixed. Electron microscopic analysis of mating mixtures revealed many cell aggregates made up of 2 to 30 cells and intercellular cytoplasmic bridges connecting two or more cells. Cells that had been transformed with purified plasmid DNA as well as transcipients isolated from mating mixtures were shown to serve as donors for further transmission of pNOB8. The plasmid undergoes extensive genetic variations, since deletions and insertions were frequently observed in plasmid preparations from the donor strain and from mating mixtures.
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- Brisson-Noël A., Arthur M., Courvalin P. Evidence for natural gene transfer from gram-positive cocci to Escherichia coli. J Bacteriol. 1988 Apr;170(4):1739–1745. doi: 10.1128/jb.170.4.1739-1745.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brock T. D., Brock K. M., Belly R. T., Weiss R. L. Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Mikrobiol. 1972;84(1):54–68. doi: 10.1007/BF00408082. [DOI] [PubMed] [Google Scholar]
- Davies J. Inactivation of antibiotics and the dissemination of resistance genes. Science. 1994 Apr 15;264(5157):375–382. doi: 10.1126/science.8153624. [DOI] [PubMed] [Google Scholar]
- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Frost L. S., Ippen-Ihler K., Skurray R. A. Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol Rev. 1994 Jun;58(2):162–210. doi: 10.1128/mr.58.2.162-210.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grogan D., Palm P., Zillig W. Isolate B12, which harbours a virus-like element, represents a new species of the archaebacterial genus Sulfolobus, Sulfolobus shibatae, sp. nov. Arch Microbiol. 1990;154(6):594–599. doi: 10.1007/BF00248842. [DOI] [PubMed] [Google Scholar]
- Heinemann J. A., Sprague G. F., Jr Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast. Nature. 1989 Jul 20;340(6230):205–209. doi: 10.1038/340205a0. [DOI] [PubMed] [Google Scholar]
- Kondo S., Yamagishi A., Oshima T. A physical map of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius 7 chromosome. J Bacteriol. 1993 Mar;175(5):1532–1536. doi: 10.1128/jb.175.5.1532-1536.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lessl M., Lanka E. Common mechanisms in bacterial conjugation and Ti-mediated T-DNA transfer to plant cells. Cell. 1994 May 6;77(3):321–324. doi: 10.1016/0092-8674(94)90146-5. [DOI] [PubMed] [Google Scholar]
- Mazodier P., Davies J. Gene transfer between distantly related bacteria. Annu Rev Genet. 1991;25:147–171. doi: 10.1146/annurev.ge.25.120191.001051. [DOI] [PubMed] [Google Scholar]
- Meile L., Abendschein P., Leisinger T. Transduction in the archaebacterium Methanobacterium thermoautotrophicum Marburg. J Bacteriol. 1990 Jun;172(6):3507–3508. doi: 10.1128/jb.172.6.3507-3508.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mevarech M., Werczberger R. Genetic transfer in Halobacterium volcanii. J Bacteriol. 1985 Apr;162(1):461–462. doi: 10.1128/jb.162.1.461-462.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenshine I., Tchelet R., Mevarech M. The mechanism of DNA transfer in the mating system of an archaebacterium. Science. 1989 Sep 22;245(4924):1387–1389. doi: 10.1126/science.2818746. [DOI] [PubMed] [Google Scholar]
- Schleper C., Kubo K., Zillig W. The particle SSV1 from the extremely thermophilic archaeon Sulfolobus is a virus: demonstration of infectivity and of transfection with viral DNA. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7645–7649. doi: 10.1073/pnas.89.16.7645. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schleper C., Röder R., Singer T., Zillig W. An insertion element of the extremely thermophilic archaeon Sulfolobus solfataricus transposes into the endogenous beta-galactosidase gene. Mol Gen Genet. 1994 Apr;243(1):91–96. doi: 10.1007/BF00283880. [DOI] [PubMed] [Google Scholar]
- Sikorski R. S., Michaud W., Levin H. L., Boeke J. D., Hieter P. Trans-kingdom promiscuity. Nature. 1990 Jun 14;345(6276):581–582. doi: 10.1038/345581b0. [DOI] [PubMed] [Google Scholar]
- Willetts N., Skurray R. The conjugation system of F-like plasmids. Annu Rev Genet. 1980;14:41–76. doi: 10.1146/annurev.ge.14.120180.000353. [DOI] [PubMed] [Google Scholar]
- Willetts N., Wilkins B. Processing of plasmid DNA during bacterial conjugation. Microbiol Rev. 1984 Mar;48(1):24–41. doi: 10.1128/mr.48.1.24-41.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woese C. R., Kandler O., Wheelis M. L. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4576–4579. doi: 10.1073/pnas.87.12.4576. [DOI] [PMC free article] [PubMed] [Google Scholar]