In one of the bedroom scenes, Paul Rudd's farts were unscripted. The crew were not amused by this but Leslie Mann stayed in the moment and improvised her outraged reaction.
The two girls playing Pete's & Debbie's children are actually the daughters of Leslie Mann & Judd Apatow. They were told to act as they normally would around each other, for instance Charlotte (Iris Aptow) performing her improvised skit about a haunted cow.
This features the first live performance by Graham Parker and The Rumour in 30 years. A lot of the band brought their children for the performance as they had never seen their fathers on stage.
Graham Parker's involvement with the film came about when one of Judd Apatow's friends mentioned to him that Parker had a blog and the most recent entry was about trying to get his music into films. The blog entry even had the sentence "Are you out there, Judd Apatow?"
In real life, Maude Apatow really did get obsessed with Lost (2004) and watched the entire series in a few weeks.
Billie Joe Armstrong: from Green Day mentions that it's a pretty big deal when Glee buys a song. "Good Riddance (Time of Your Life)" by Green Day was purchased by Glee but never aired.
Bill Hader: another actor from Knocked Up (2007), but as a different character and it's uncredited. He is Megan Fox's customer.