Jump to content

Isoleucine—tRNA ligase

From Wikipedia, the free encyclopedia
(Redirected from Isoleucine-tRNA ligase)
Isoleucine—tRNA ligase
Identifiers
EC no.6.1.1.5
CAS no.9030-96-0
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

In enzymology, an isoleucine—tRNA ligase (EC 6.1.1.5) is an enzyme that catalyzes the chemical reaction

ATP + L-isoleucine + tRNAIle AMP + diphosphate + L-isoleucyl-tRNAIle

The 3 substrates of this enzyme are ATP, L-isoleucine, and tRNA(Ile), whereas its 3 products are AMP, diphosphate, and L-isoleucyl-tRNA(Ile).

This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-isoleucine:tRNAIle ligase (AMP-forming). Other names in common use include isoleucyl-tRNA synthetase, isoleucyl-transfer ribonucleate synthetase, isoleucyl-transfer RNA synthetase, isoleucine-transfer RNA ligase, isoleucine-tRNA synthetase, and isoleucine translase. This enzyme participates in valine, leucine and isoleucine biosynthesis and aminoacyl-trna biosynthesis.

Structural studies

[edit]

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1FFY, 1JZQ, 1JZS, 1QU2, 1QU3, 1UDZ, 1UE0, 1WK8, 1WNY, and 1WNZ.

References

[edit]
  • ALLEN EH, GLASSMAN E, SCHWEET RS (1960). "Incorporation of amino acids into ribonucleic acid. I. The role of activating enzymes". J. Biol. Chem. 235 (4): 1061–7. doi:10.1016/S0021-9258(18)69479-7. PMID 13792726.
  • Berg P, Bergmann FH, Ofengand EJ, Dieckmann M (1961). "The enzymic synthesis of amino acyl derivatives of ribonucleic acid I. The mechanism of leucyl-, valyl-, isoleucyl- and methionyl ribonucleic acid formation". J. Biol. Chem. 236: 1726–1734. doi:10.1016/S0021-9258(19)63293-X.
  • Bergmann FH, Berg P, Dieckmann M (1961). "The enzymic synthesis of amino acyl derivatives of ribonucleic acid II. The preparation of leucyl-, valyl-, isoleucyl- and methionyl ribonucleic acid synthetases from Escherichia coli". J. Biol. Chem. 236: 1735–1740. doi:10.1016/S0021-9258(19)63294-1.