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File:MeDIP.svg

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English: Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches for fragments that are methylated (IP fraction). This IP fraction can become the input sample to one of two DNA detection methods: array hybridization using high-density microarrays (A) or high-throughput sequencing using the latest in sequencing technology (B). Output from these methods are then analyzed for methylation patterns to answer the biological question.
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Source This file was derived from: Medip-final-3.jpg
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This is a retouched picture, which means that it has been digitally altered from its original version. Modifications: svg. The original can be viewed here: Medip-final-3.jpg.

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  • File:Medip-final-3.jpg licensed with PD-self
    • 2009-03-03T06:16:21Z Gavinha 2268x1868 (357388 Bytes) {{Information |Description={{en|1=Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches

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15 June 2013

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Date/TimeThumbnailDimensionsUserComment
current05:11, 15 June 2013Thumbnail for version as of 05:11, 15 June 20131,150 × 1,000 (648 KB)Shakiestone== {{int:filedesc}} == {{Information |Description={{en|1=Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches for...

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