PTPN12

PTPN12
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	5J8R
Identifiers
Aliases	PTPN12, PTP-PEST, PTPG1, protein tyrosine phosphatase, non-receptor type 12, protein tyrosine phosphatase non-receptor type 12
External IDs	OMIM: 600079; MGI: 104673; HomoloGene: 37691; GeneCards: PTPN12; OMA:PTPN12 - orthologs
Gene location (Human)
Chr.	Chromosome 7 (human)
End	77,640,069 bp
Gene location (Mouse)
Chr.	Chromosome 5 (mouse)
End	21,055,911 bp
RNA expression pattern
	Top expressed in
	gonad; ; epithelium of colon; ; monocyte; ; right lung; ; tibial nerve; ; Achilles tendon; ; lower lobe of lung; ; upper lobe of lung; ; upper lobe of left lung; ; subcutaneous adipose tissue;
	Top expressed in
	tail of embryo; ; Gonadal ridge; ; left lung lobe; ; genital tubercle; ; stroma of bone marrow; ; trigeminal ganglion; ; abdominal wall; ; otic placode; ; hair follicle; ; dermis;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	SH3 domain binding; phosphatase activity; protein binding; hydrolase activity; non-membrane spanning protein tyrosine phosphatase activity; phosphoprotein phosphatase activity; protein tyrosine phosphatase activity;
Cellular component	podosome; cell junction; cell projection; focal adhesion; cytoplasm; cytosol; nucleoplasm;
Biological process	tissue regeneration; dephosphorylation; protein dephosphorylation; regulation of epidermal growth factor receptor signaling pathway; peptidyl-tyrosine dephosphorylation; cellular response to epidermal growth factor stimulus; ERBB2 signaling pathway; negative regulation of ERBB signaling pathway; negative regulation of platelet-derived growth factor receptor-beta signaling pathway; cellular response to cytokine stimulus;
	Sources:Amigo / QuickGO
Orthologs
	5782
	19248
	ENSG00000127947
	ENSMUSG00000028771
	Q05209
	P35831
	NM_001131008; NM_001131009; NM_002835
	NM_011203; NM_001356590; NM_001356591; NM_001356592
	NP_001124480; NP_001124481; NP_002826
	NP_035333; NP_001343519; NP_001343520; NP_001343521
	Wikidata
View/Edit Human	View/Edit Mouse

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Tyrosine-protein phosphatase non-receptor type 12 is an enzyme that in humans is encoded by the PTPN12 gene.^[5]^[6]

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein–protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.^[6]

Interactions

PTPN12 has been shown to interact with BCAR1,^[7]^[8]^[9]^[10] Grb2,^[11] PSTPIP1,^[12] TGFB1I1,^[13] Paxillin^[14]^[15]^[16] and SHC1.^[17]^[18]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000127947 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000028771 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Takekawa M, Itoh F, Hinoda Y, Adachi M, Ariyama T, Inazawa J, Imai K, Yachi A (March 1994). "Chromosomal localization of the protein tyrosine phosphatase G1 gene and characterization of the aberrant transcripts in human colon cancer cells". FEBS Lett. 339 (3): 222–8. doi:10.1016/0014-5793(94)80420-6. PMID 7509295. S2CID 4885570.
^ ^a ^b "Entrez Gene: PTPN12 protein tyrosine phosphatase, non-receptor type 12".
^ Lin, Yi; Ceacareanu Alice Corina; Hassid Aviv (August 2003). "Nitric oxide-induced inhibition of aortic smooth muscle cell motility: role of PTP-PEST and adaptor proteins p130cas and Crk". Am. J. Physiol. Heart Circ. Physiol. 285 (2): H710–21. doi:10.1152/ajpheart.01127.2002. ISSN 0363-6135. PMID 12714323. S2CID 42587789.
^ Garton, A J; Burnham M R; Bouton A H; Tonks N K (August 1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition". Oncogene. 15 (8): 877–85. doi:10.1038/sj.onc.1201279. ISSN 0950-9232. PMID 9285683.
^ Côté, J F; Charest A; Wagner J; Tremblay M L (September 1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry. 37 (38): 13128–37. doi:10.1021/bi981259l. ISSN 0006-2960. PMID 9748319.
^ Garton, A J; Flint A J; Tonks N K (November 1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST". Mol. Cell. Biol. 16 (11): 6408–18. doi:10.1128/MCB.16.11.6408. ISSN 0270-7306. PMC 231642. PMID 8887669.
^ Charest, A; Wagner J; Kwan M; Tremblay M L (April 1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2". Oncogene. 14 (14): 1643–51. doi:10.1038/sj.onc.1201008. ISSN 0950-9232. PMID 9135065.
^ Dowbenko, D; Spencer S; Quan C; Lasky L A (January 1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein". J. Biol. Chem. 273 (2): 989–96. doi:10.1074/jbc.273.2.989. ISSN 0021-9258. PMID 9422760.
^ Nishiya, N; Iwabuchi Y; Shibanuma M; Côté J F; Tremblay M L; Nose K (April 1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain". J. Biol. Chem. 274 (14): 9847–53. doi:10.1074/jbc.274.14.9847. ISSN 0021-9258. PMID 10092676.
^ Shen, Y; Lyons P; Cooley M; Davidson D; Veillette A; Salgia R; Griffin J D; Schaller M D (January 2000). "The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo". J. Biol. Chem. 275 (2): 1405–13. doi:10.1074/jbc.275.2.1405. ISSN 0021-9258. PMID 10625692.
^ Côté, J F; Turner C E; Tremblay M L (July 1999). "Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST". J. Biol. Chem. 274 (29): 20550–60. doi:10.1074/jbc.274.29.20550. ISSN 0021-9258. PMID 10400685.
^ Shen, Y; Schneider G; Cloutier J F; Veillette A; Schaller M D (March 1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". J. Biol. Chem. 273 (11): 6474–81. doi:10.1074/jbc.273.11.6474. ISSN 0021-9258. PMID 9497381.
^ Habib, T; Herrera R; Decker S J (October 1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase". J. Biol. Chem. 269 (41): 25243–6. doi:10.1016/S0021-9258(18)47237-7. ISSN 0021-9258. PMID 7929214.
^ Charest, A; Wagner J; Jacob S; McGlade C J; Tremblay M L (April 1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity". J. Biol. Chem. 271 (14): 8424–9. doi:10.1074/jbc.271.14.8424. ISSN 0021-9258. PMID 8626541.

Takekawa M, Itoh F, Hinoda Y, et al. (1993). "Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein-tyrosine-phosphatase". Biochem. Biophys. Res. Commun. 189 (2): 1223–30. doi:10.1016/0006-291X(92)92335-U. PMID 1472029.
Yi T, Cleveland JL, Ihle JN (1991). "Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification". Blood. 78 (9): 2222–8. doi:10.1182/blood.V78.9.2222.2222. PMID 1932742.
Garton AJ, Tonks NK (1994). "PTP-PEST: a protein tyrosine phosphatase regulated by serine phosphorylation". EMBO J. 13 (16): 3763–71. doi:10.1002/j.1460-2075.1994.tb06687.x. PMC 395289. PMID 7520867.
Habib T, Herrera R, Decker SJ (1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase". J. Biol. Chem. 269 (41): 25243–6. doi:10.1016/S0021-9258(18)47237-7. PMID 7929214.
Yang Q, Co D, Sommercorn J, Tonks NK (1993). "Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase". J. Biol. Chem. 268 (23): 17650. doi:10.1016/S0021-9258(19)85383-8. PMID 8349645.
Yang Q, Co D, Sommercorn J, Tonks NK (1993). "Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase". J. Biol. Chem. 268 (9): 6622–8. doi:10.1016/S0021-9258(18)53296-8. PMID 8454633.
Charest A, Wagner J, Jacob S, et al. (1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity". J. Biol. Chem. 271 (14): 8424–9. doi:10.1074/jbc.271.14.8424. PMID 8626541.
Garton AJ, Flint AJ, Tonks NK (1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST". Mol. Cell. Biol. 16 (11): 6408–18. doi:10.1128/MCB.16.11.6408. PMC 231642. PMID 8887669.
Charest A, Wagner J, Kwan M, Tremblay ML (1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2". Oncogene. 14 (14): 1643–51. doi:10.1038/sj.onc.1201008. PMID 9135065.
Garton AJ, Burnham MR, Bouton AH, Tonks NK (1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition". Oncogene. 15 (8): 877–85. doi:10.1038/sj.onc.1201279. PMID 9285683.
Dowbenko D, Spencer S, Quan C, Lasky LA (1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein". J. Biol. Chem. 273 (2): 989–96. doi:10.1074/jbc.273.2.989. PMID 9422760.
Shen Y, Schneider G, Cloutier JF, et al. (1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". J. Biol. Chem. 273 (11): 6474–81. doi:10.1074/jbc.273.11.6474. PMID 9497381.
Côté JF, Charest A, Wagner J, Tremblay ML (1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry. 37 (38): 13128–37. doi:10.1021/bi981259l. PMID 9748319.
Wu Y, Dowbenko D, Lasky LA (1998). "PSTPIP 2, a second tyrosine phosphorylated, cytoskeletal-associated protein that binds a PEST-type protein-tyrosine phosphatase". J. Biol. Chem. 273 (46): 30487–96. doi:10.1074/jbc.273.46.30487. PMID 9804817.
Sanger Centre, The; Washington University Genome Sequencing Cente, The (1999). "Toward a complete human genome sequence". Genome Res. 8 (11): 1097–108. doi:10.1101/gr.8.11.1097. PMID 9847074.
Li J, Nishizawa K, An W, et al. (1999). "A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion". EMBO J. 17 (24): 7320–36. doi:10.1093/emboj/17.24.7320. PMC 1171078. PMID 9857189.
Garton AJ, Tonks NK (1999). "Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST". J. Biol. Chem. 274 (6): 3811–8. doi:10.1074/jbc.274.6.3811. PMID 9920935.
Angers-Loustau A, Côté JF, Charest A, et al. (1999). "Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts". J. Cell Biol. 144 (5): 1019–31. doi:10.1083/jcb.144.5.1019. PMC 2148201. PMID 10085298.
Nishiya N, Iwabuchi Y, Shibanuma M, et al. (1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain". J. Biol. Chem. 274 (14): 9847–53. doi:10.1074/jbc.274.14.9847. PMID 10092676.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000127947 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000028771 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7509295-5] Takekawa M, Itoh F, Hinoda Y, Adachi M, Ariyama T, Inazawa J, Imai K, Yachi A (March 1994). "Chromosomal localization of the protein tyrosine phosphatase G1 gene and characterization of the aberrant transcripts in human colon cancer cells". FEBS Lett. 339 (3): 222–8. doi:10.1016/0014-5793(94)80420-6. PMID 7509295. S2CID 4885570.

[entrez-6] "Entrez Gene: PTPN12 protein tyrosine phosphatase, non-receptor type 12".

[pmid12714323-7] Lin, Yi; Ceacareanu Alice Corina; Hassid Aviv (August 2003). "Nitric oxide-induced inhibition of aortic smooth muscle cell motility: role of PTP-PEST and adaptor proteins p130cas and Crk". Am. J. Physiol. Heart Circ. Physiol. 285 (2): H710–21. doi:10.1152/ajpheart.01127.2002. ISSN 0363-6135. PMID 12714323. S2CID 42587789.

[pmid9285683-8] Garton, A J; Burnham M R; Bouton A H; Tonks N K (August 1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition". Oncogene. 15 (8): 877–85. doi:10.1038/sj.onc.1201279. ISSN 0950-9232. PMID 9285683.

[pmid9748319-9] Côté, J F; Charest A; Wagner J; Tremblay M L (September 1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry. 37 (38): 13128–37. doi:10.1021/bi981259l. ISSN 0006-2960. PMID 9748319.

[pmid8887669-10] Garton, A J; Flint A J; Tonks N K (November 1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST". Mol. Cell. Biol. 16 (11): 6408–18. doi:10.1128/MCB.16.11.6408. ISSN 0270-7306. PMC 231642. PMID 8887669.

[pmid9135065-11] Charest, A; Wagner J; Kwan M; Tremblay M L (April 1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2". Oncogene. 14 (14): 1643–51. doi:10.1038/sj.onc.1201008. ISSN 0950-9232. PMID 9135065.

[pmid9422760-12] Dowbenko, D; Spencer S; Quan C; Lasky L A (January 1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein". J. Biol. Chem. 273 (2): 989–96. doi:10.1074/jbc.273.2.989. ISSN 0021-9258. PMID 9422760.

[pmid10092676-13] Nishiya, N; Iwabuchi Y; Shibanuma M; Côté J F; Tremblay M L; Nose K (April 1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain". J. Biol. Chem. 274 (14): 9847–53. doi:10.1074/jbc.274.14.9847. ISSN 0021-9258. PMID 10092676.

[pmid10625692-14] Shen, Y; Lyons P; Cooley M; Davidson D; Veillette A; Salgia R; Griffin J D; Schaller M D (January 2000). "The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo". J. Biol. Chem. 275 (2): 1405–13. doi:10.1074/jbc.275.2.1405. ISSN 0021-9258. PMID 10625692.

[pmid10400685-15] Côté, J F; Turner C E; Tremblay M L (July 1999). "Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST". J. Biol. Chem. 274 (29): 20550–60. doi:10.1074/jbc.274.29.20550. ISSN 0021-9258. PMID 10400685.

[pmid9497381-16] Shen, Y; Schneider G; Cloutier J F; Veillette A; Schaller M D (March 1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". J. Biol. Chem. 273 (11): 6474–81. doi:10.1074/jbc.273.11.6474. ISSN 0021-9258. PMID 9497381.

[pmid7929214-17] Habib, T; Herrera R; Decker S J (October 1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase". J. Biol. Chem. 269 (41): 25243–6. doi:10.1016/S0021-9258(18)47237-7. ISSN 0021-9258. PMID 7929214.

[pmid8626541-18] Charest, A; Wagner J; Jacob S; McGlade C J; Tremblay M L (April 1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity". J. Biol. Chem. 271 (14): 8424–9. doi:10.1074/jbc.271.14.8424. ISSN 0021-9258. PMID 8626541.

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Interactions

References

Further reading