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28S ribosomal RNA

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Mitochondrial 16S and nuclear 28S ribosomal DNA phylogenies of three species of mollusks belonging to the Waldo genus.

28S ribosomal RNA is the structural ribosomal RNA (rRNA) for the large component, or large subunit (LSU) of eukaryotic cytoplasmic ribosomes, and thus one of the basic components of all eukaryotic cells.[1] It is the eukaryotic nuclear homologue of the prokaryotic 23S ribosomal RNA.

The genes coding for 28S rRNA are referred to as 28S rDNA. The comparison of the sequences from these genes are sometimes used in molecular analysis to construct phylogenetic trees, for example in Protists,[2] Fungi,[3] Insects,[4] Tardigrades,[5] and Vertebrates.[6] [7]

References

  1. ^ Lodish, H. (1995). Molecular Cell Biology, 3rd ed. Freeman.
  2. ^ Baroin, A.; Perasso, R.; Qu, L. H.; Brugerolle, G.; Bachellerie, J. P.; Adoutte, A. (1988-05-01). "Partial phylogeny of the unicellular eukaryotes based on rapid sequencing of a portion of 28S ribosomal RNA". Proceedings of the National Academy of Sciences. 85 (10): 3474–3478. ISSN 0027-8424. PMC 280234. PMID 3368456.
  3. ^ James, Timothy Y.; Kauff, Frank; Schoch, Conrad L.; Matheny, P. Brandon; Hofstetter, Valérie; Cox, Cymon J.; Celio, Gail; Gueidan, Cécile; Fraker, Emily. "Reconstructing the early evolution of Fungi using a six-gene phylogeny". Nature. 443 (7113): 818–822. doi:10.1038/nature05110.
  4. ^ Whiting, Michael F.; Carpenter, James C.; Wheeler, Quentin D.; Wheeler, Ward C. (1997-03-01). "The Strepsiptera Problem: Phylogeny of the Holometabolous Insect Orders Inferred from 18S and 28S Ribosomal DNA Sequences and Morphology". Systematic Biology. 46 (1): 1–68. doi:10.1093/sysbio/46.1.1. ISSN 1063-5157. PMID 11975347.
  5. ^ Jørgensen, Aslak; Faurby, Søren; Hansen, Jesper G.; Møbjerg, Nadja; Kristensen, Reinhardt M. (2010-03-01). "Molecular phylogeny of Arthrotardigrada (Tardigrada)". Molecular Phylogenetics and Evolution. 54 (3): 1006–1015. doi:10.1016/j.ympev.2009.10.006.
  6. ^ Le, Hoc Lanh Vân; Lecointre, Guillaume; Perasso, Roland (1993-03-01). "A 28S rRNA-Based Phylogeny of the Gnathostomes: First Steps in the Analysis of Conflict and Congruence with Morphologically Based Cladograms". Molecular Phylogenetics and Evolution. 2 (1): 31–51. doi:10.1006/mpev.1993.1005.
  7. ^ Mallatt, Jon; Sullivan, Jack (December 1998). "28S and 18S rDNA sequences support the monophyly of lampreys and hagfishes". Molecular Biology and Evolution. 15 (12): 1706–18. doi:10.1093/oxfordjournals.molbev.a025897. PMID 9866205.