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Blood plasma is a light amber-colored liquid component of blood in which blood cells are absent, but which contains proteins and other constituents of whole blood in suspension. It makes up about 55% of the body's total blood volume.^[1] It is the intravascular part of extracellular fluid (all body fluid outside cells). It is mostly water (up to 95% by volume), and contains important dissolved proteins (6–8%; e.g., serum albumins, globulins, and fibrinogen),^[2] glucose, clotting factors, electrolytes (Na⁺
, Ca²⁺
, Mg²⁺
, HCO₃⁻, Cl⁻
, etc.), hormones, carbon dioxide (plasma being the main medium for excretory product transportation), and oxygen.^[3] It plays a vital role in an intravascular osmotic effect that keeps electrolyte concentration balanced and protects the body from infection and other blood-related disorders.^[4]

Blood plasma can be separated from whole blood through blood fractionation, by adding an anticoagulant to a tube filled with blood, which is spun in a centrifuge until the blood cells fall to the bottom of the tube. The blood plasma is then poured or drawn off.^[5] For point-of-care testing applications, plasma can be extracted from whole blood via filtration^[6] or via agglutination^[7] to allow for rapid testing of specific biomarkers. Blood plasma has a density of approximately 1,025 kg/m³ (1.025 g/ml).^[8] Blood serum is blood plasma without clotting factors.^[5] Plasmapheresis is a medical therapy that involves blood plasma extraction, treatment, and reintegration.

Fresh frozen plasma is on the WHO Model List of Essential Medicines, the most important medications needed in a basic health system.^[9] It is of critical importance in the treatment of many types of trauma which result in blood loss, and is therefore kept stocked universally in all medical facilities capable of treating trauma (e.g., trauma centers, hospitals, and ambulances) or that pose a risk of patient blood loss such as surgical suite facilities ^[10]

Volume

Reference ranges for blood tests, showing normal mass concentration of blood plasma constituents

The same information, shown in molarity rather than mass

Blood plasma volume may be expanded by or drained to extravascular fluid when there are changes in Starling forces across capillary walls. For example, when blood pressure drops in circulatory shock, Starling forces drive fluid into the interstitium, causing third spacing.^[11]

Standing still for a prolonged period will cause an increase in transcapillary hydrostatic pressure. As a result, approximately 12% of blood plasma volume will cross into the extravascular compartment. This plasma shift causes an increase in hematocrit, serum total protein, blood viscosity and, as a result of increased concentration of coagulation factors, it causes orthostatic hypercoagulability.^[12]

Plasma proteins

Albumins

Serum albumins are the most common plasma proteins, and they are responsible for maintaining the osmotic pressure of the blood. Without albumins, the consistency of blood would be closer to that of water. The increased viscosity of blood prevents fluid from entering the bloodstream from outside the capillaries. Albumins are produced in the liver, assuming the absence of a hepatocellular deficiency.^[13]

Globulins

The second most common type of protein in the blood plasma are globulins. Important globulins include immunoglobins which are important for the immune system and transport hormones and other compounds around the body. There are three main types of globulins. Alpha-1 and Alpha-2 globulins are formed in the liver and play an important role in mineral transport and the inhibition of blood coagulation.^[14] An example of beta globulin found in blood plasma includes low-density lipoproteins (LDL) which are responsible for transporting fat to the cells for steroid and membrane synthesis.^[15] Gamma globulin, better known as immunoglobulins, are produced by plasma B cells, and provides the human body with a defense system against invading pathogens and other immune diseases.^[16]

Fibrinogen

Fibrinogen proteins make up most of the remaining proteins in the blood. Fibrinogens are responsible for clotting blood to help prevent blood loss.^[17]

Color

Plasma is normally yellow due to bilirubin, carotenoids, hemoglobin, and transferrin.^[18] In abnormal cases, plasma can have varying shades of orange, green, or brown. The green color can be due to ceruloplasmin or sulfhemoglobin. The latter may form due to medicines that are able to form sulfonamides once ingested.^[19] A dark brown or reddish color can appear due to hemolysis, in which methemoglobin is released from broken blood cells.^[20] Plasma is normally relatively transparent, but sometimes it can be opaque. Opaqueness is typically due to elevated content of lipids like cholesterol and triglycerides.^[21]

Plasma vs. serum in medical diagnostics

Blood plasma and blood serum are often used in blood tests. Tests can be done on plasma, serum or both.^[22] In addition, some tests have to be done with whole blood, such as the determination of the amount of blood cells in blood via flow cytometry.^[23]


Some of the benefits of plasma over serum	Some of the benefits of serum over plasma
Plasma preparation is quick, as it is not coagulated. Serum sample preparation requires about 30 minutes of waiting time before it can be centrifuged and then analyzed.^[22] However, coagulation can be hastened down to a few minutes by adding thrombin or similar agents to the serum sample.^[24]	Plasma preparation requires the addition of anticoagulants, which can cause expected and unexpected measurement errors. For example, anticoagulant salts can add extra cations like NH₄⁺, Li⁺, Na⁺ and K⁺ to the sample,^[22] or impurities like lead and aluminum.^[25] Chelator anticoagulants like EDTA and citrate salts work by binding calcium (see carboxyglutamic acid), but they may also bind other ions. Even if such ions are not the analytes, chelators can interfere with enzyme activity measurements. For example, EDTA binds zinc ions, which alkaline phosphatases need as cofactors. Thus, phosphatase activity cannot be measured if EDTA is used.^[22]
Compared to serum, 15–20% larger volume of plasma can be obtained from a blood sample of certain size. Serum lacks some proteins that partake in coagulation and increase the sample volume.^[22]	An unknown volume of anticoagulants can be added to a plasma sample by accident, which may ruin the sample as the analyte concentration is changed by an unknown amount.^[25]
Serum preparation can cause measurement errors by increasing or decreasing the concentration of the analyte that is meant to be measured. For example, during coagulation, blood cells consume blood glucose and platelets increase the sample content of compounds like potassium, phosphates and aspartate transaminase by secreting them. Glucose or these other compounds may be the analytes.^[22]	No anticoagulants are added to serum samples, which decreases the preparation cost of the samples relative to plasma samples.^[25]
	Plasma samples can form tiny clots if the added anticoagulant is not properly mixed with the sample. Non-uniform samples can cause measurement errors.^[25]

History

Plasma was already well known when described by William Harvey in de Motu Cordis in 1628, but knowledge of it probably dates as far back as Vesalius (1514–1564). The discovery of fibrinogen by William Henson, c. 1770,^[26] made it easier to study plasma, as ordinarily, upon coming in contact with a foreign surface – something other than the vascular endothelium – clotting factors become activated and clotting proceeds rapidly, trapping RBCs etc. in the plasma and preventing separation of plasma from the blood. Adding citrate and other anticoagulants is a relatively recent advance. Upon the formation of a clot, the remaining clear fluid (if any) is blood serum, which is essentially plasma without the clotting factors^[27]

The use of blood plasma as a substitute for whole blood and for transfusion purposes was proposed in March 1918, in the correspondence columns of the British Medical Journal, by Gordon R. Ward. "Dried plasmas" in powder or strips of material format were developed and first used in World War II. Prior to the United States' involvement in the war, liquid plasma and whole blood were used.^[28]

The origin of plasmapheresis

Dr. José Antonio Grifols Lucas, a scientist from Vilanova i la Geltrú, Spain,^[29] founded Laboratorios Grifols in 1940.^[30] Dr. Grifols pioneered a first-of-its-kind technique called plasmapheresis,^[30] where a donor's red blood cells would be returned to the donor's body almost immediately after the separation of the blood plasma. This technique is still in practice today, almost 80 years later. In 1945, Dr. Grifols opened the world's first plasma donation center.^[29]

Blood for Britain

The "Blood for Britain" program during the early 1940s was quite successful (and popular in the United States) based on Charles Drew's contribution. A large project began in August 1940 to collect blood in New York City hospitals for the export of plasma to Britain. Drew was appointed medical supervisor of the "Plasma for Britain" project. His notable contribution at this time was to transform the test tube methods of many blood researchers into the first successful mass production techniques.^[31]

Nevertheless, the decision was made to develop a dried plasma package for the armed forces as it would reduce breakage and make the transportation, packaging, and storage much simpler.^[32] The resulting dried plasma package came in two tin cans containing 400 cc bottles. One bottle contained enough distilled water to reconstitute the dried plasma contained within the other bottle. In about three minutes, the plasma would be ready to use and could stay fresh for around four hours. The Blood for Britain program operated successfully for five months, with total collections of almost 15,000 people donating blood, and with over 5,500 vials of blood plasma.^[33]

Following the Supplying Blood Plasma to England project, Drew was named director of the Red Cross blood bank and assistant director of the National Research Council, in charge of blood collection for the United States Army and Navy. Drew argued against the armed forces directive that blood/plasma was to be separated by the race of the donor. Drew insisted that there was no racial difference in human blood and that the policy would lead to needless deaths as soldiers and sailors were required to wait for "same race" blood.^[34]

By the end of the war the American Red Cross had provided enough blood for over six million plasma packages. Most of the surplus plasma was returned to the United States for civilian use. Serum albumin replaced dried plasma for combat use during the Korean War.^[32]

Plasma donation

Plasma as a blood product prepared from blood donations is used in blood transfusions, typically as fresh frozen plasma (FFP) or Plasma Frozen within 24 hours after phlebotomy (PF24). When donating whole blood or packed red blood cell (PRBC) transfusions, O- is the most desirable and is considered a "universal donor," since it has neither A nor B antigens and can be safely transfused to most recipients. Type AB+ is the "universal recipient" type for PRBC donations. However, for plasma the situation is somewhat reversed. Blood donation centers will sometimes collect only plasma from AB donors through apheresis, as their plasma does not contain the antibodies that may cross react with recipient antigens. As such, AB is often considered the "universal donor" for plasma. Special programs exist just to cater to the male AB plasma donor, because of concerns about transfusion related acute lung injury (TRALI) and female donors who may have higher leukocyte antibodies.^[35] However, some studies show an increased risk of TRALI despite increased leukocyte antibodies in women who have been pregnant.^[36]

United Kingdom

Following fears of variant Creutzfeldt-Jakob disease (vCJD) being spread through the blood supply, the British government began to phase out blood plasma from U.K. donors and by the end of 1999 had imported all blood products made with plasma from the United States.^[37] In 2002, the British government purchased Life Resources Incorporated, an American blood supply company, to import plasma.^[38] The company became Plasma Resources UK (PRUK) which owned Bio Products Laboratory. In 2013, the British government sold an 80% stake in PRUK to American hedge fund Bain Capital, in a deal estimated to be worth £200 million. The sale was met with criticism in the UK.^[39] In 2009, the U.K. stopped importing plasma from the United States, as it was no longer a viable option due to regulatory and jurisdictional challenges.^[40]

At present (2024), blood donated in the United Kingdom is used by UK Blood Services for the manufacture of plasma blood components (Fresh Frozen Plasma (FFP) and cryoprecipitate). However, plasma from UK donors is still not used for the commercial manufacture of fractionated plasma medicines.^[41]

Synthetic blood plasma

Simulated body fluid (SBF) is a solution having a similar ion concentration to that of human blood plasma. SBF is normally used for the surface modification of metallic implants, and more recently in gene delivery application.^[42]

References

^ Dennis O'Neil (1999). "Blood Components". Palomar College. Archived from the original on June 5, 2013.
^ Tuskegee University (May 29, 2013). "Chapter 9 Blood". tuskegee.edu. Archived from the original on December 28, 2013.
^ Mathew, Joscilin; Sankar, Parvathy; varacallo, Matthew (2024). "Physiology, blood plasma". Europe PMC. PMID 30285399. Retrieved January 23, 2024.
^ "Ways to Keep Your Blood Plasma Healthy". BloodBanker. Archived from the original on November 1, 2013. Retrieved November 10, 2011.
^ ^a ^b Maton A, Hopkins J, McLaughlin CW, Johnson S, Warner MQ, LaHart D, Wright JD (1993). Human Biology and Health. Englewood Cliffs, New Jersey: Prentice Hall. ISBN 0-13-981176-1.
^ Tripathi S, Kumar V, Prabhakar A, Joshi S, Agrawal A (2015). "Passive blood plasma separation at the microscale: a review of design principles and microdevices". J. Micromech. Microeng. 25 (8): 083001. Bibcode:2015JMiMi..25h3001T. doi:10.1088/0960-1317/25/8/083001. S2CID 138153068.
^ Guo W, Hansson J, van der Wijngaart W (May 2020). "Synthetic Paper Separates Plasma from Whole Blood with Low Protein Loss". Analytical Chemistry. 92 (9): 6194–6199. doi:10.1021/acs.analchem.0c01474. PMID 32323979.
^ Shmukler M (2004). Elert G (ed.). "Density of blood". The Physics Factbook. Archived from the original on December 9, 2021. Retrieved January 23, 2022.
^ "19th WHO Model List of Essential Medicines (April 2015)" (PDF). WHO. April 2015. Archived (PDF) from the original on April 28, 2019. Retrieved May 10, 2015.
^ Soffer D (2008). "Usage of Blood Products in Multiple Casualty Incidents: The Experience of a Level I Trauma Center in Israel". Archives of Surgery. 143 (10): 983–989. doi:10.1001/archsurg.143.10.983. PMID 18936378. Retrieved January 23, 2024.
^ Dargent A, Dumargne H, Labruyère M, et al. (October 2023). "Role of the interstitium during septic shock: a key to the understanding of fluid dynamics?". Intensive Care. 11 (44): 44. doi:10.1186/s40560-023-00694-z. PMC 10565984. PMID 37817235.
^ Masoud M, Sarig G, Brenner B, Jacob G (June 2008). "Orthostatic hypercoagulability: a novel physiological mechanism to activate the coagulation system". Hypertension. 51 (6): 1545–1551. doi:10.1161/HYPERTENSIONAHA.108.112003. PMID 18413485.
^ "Albumin: Liver Function Test - Viral Hepatitis and Liver Disease". U.S. Department of Veterans Affairs. Archived from the original on September 13, 2021. Retrieved March 15, 2021.
^ "Globulins | Encyclopedia.com". www.encyclopedia.com. Archived from the original on November 29, 2021. Retrieved November 29, 2021.
^ Thomas L (October 10, 2018). Simmons H (ed.). "Blood Plasma Components and Function". News-Medical.net. Archived from the original on November 29, 2021. Retrieved November 29, 2021.
^ Biga LM, Dawson S, Harwell A, Hopkins R, Kaufmann J, LeMaster M, et al. (September 26, 2019). "18.1 Functions of Blood". Anatomy & Physiology. OpenStax. Archived from the original on November 29, 2021. Retrieved November 29, 2021 – via Oregon State University.
^ "Blood cells". Basic Biology. 2015. Archived from the original on July 18, 2021. Retrieved March 17, 2020.
^ Elkassabany NM, Meny GM, Doria RR, Marcucci C (April 2008). "Green plasma-revisited". Anesthesiology. 108 (4): 764–765. doi:10.1097/ALN.0b013e3181672668. PMID 18362615. Archived from the original on July 27, 2020. Retrieved March 21, 2020.
^ Mani A, Poornima AP, Gupta D (2019). "Greenish discoloration of plasma: Is it really a matter of concern?". Asian Journal of Transfusion Science. 13 (1): 1–2. doi:10.4103/ajts.AJTS_117_18. PMC 6580839. PMID 31360002.
^ Tesfazghi MT, McGill MR, Yarbrough ML (July 2019). "What's Causing This Dark Brown Plasma?". The Journal of Applied Laboratory Medicine. 4 (1): 125–129. doi:10.1373/jalm.2018.026633. PMID 31639715.
^ Agnihotri N, Kumar L (July 2014). "Turbid plasma donations: Need for quantification". Asian Journal of Transfusion Science. 8 (2): 78–79. doi:10.4103/0973-6247.137436. PMC 4140067. PMID 25161342.
^ ^a ^b ^c ^d ^e ^f "Use of anticoagulants in diagnostic laboratory investigations". World Health Organization. 2002. hdl:10665/65957. WHO/DIL/LAB/99.1 Rev.2.
^ Jimenez Vera E, Chew YV, Nicholson L, Burns H, Anderson P, Chen HT, et al. (2019). "Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients". PLOS ONE. 14 (5): e0217163. Bibcode:2019PLoSO..1417163J. doi:10.1371/journal.pone.0217163. PMC 6530858. PMID 31116766.
^ Kocijancic M, Cargonja J, Delic-Knezevic A (2014). "Evaluation of the BD Vacutainer(®) RST blood collection tube for routine chemistry analytes: clinical significance of differences and stability study". Biochemia Medica. 24 (3): 368–375. doi:10.11613/BM.2014.039. PMC 4210257. PMID 25351355.
^ ^a ^b ^c ^d Uges DR (October 1988). "Plasma or serum in therapeutic drug monitoring and clinical toxicology". Pharmaceutisch Weekblad. Scientific Edition. 10 (5): 185–188. doi:10.1007/BF01956868. PMID 3060834. S2CID 32330414.
^ Wintrobe MM. Blood, Pure and Eloquent: a story of discovery, of people, and of ideas.
^ Brake, Marisa; Ivanciu, Lacramioara; Maroney, Susan; Martinez, Nicolas; Mast, Alan; Westrick, Randal (March 15, 2019). "Assessing blood clotting and coagulation factors in mice". Current Protocols in Mouse Biology. 9 (2): e61. doi:10.1002/cpmo.61. PMC 6771260. PMID 30875463.
^ Pusateri, Anthony; Given, Michael; Schreiber, Martin; Spinella, Philip; Pati, Shibani; Kozar, Rosemary; Khan, Abdul; Dacorta, Joseph; Kupferer, Kevin; Prat, Nicolas; Pidcoke, Heather; Macdonald, Victor; Malloy, Wilbur; Sailliol, Anne; Cap, Andrew (April 21, 2016). "Dried plasma: state of the science and recent developments". Transfusion. 56 (S2): S128–39. doi:10.1111/trf.13580. PMID 27100749. Retrieved January 23, 2024.
^ ^a ^b "When a Dream Comes True". grifols.com. January 2015. Archived from the original on July 25, 2021. Retrieved March 21, 2020.
^ ^a ^b "Biography: J.A. Grifols". discovertheplasma.com. Archived from the original on July 25, 2021. Retrieved March 21, 2020.
^ "Father of the Blood Bank". Blood Cancer UK. Retrieved January 24, 2024.
^ ^a ^b "Home". achh.army.mil. Archived from the original on December 11, 2021. Retrieved December 9, 2021.
^ Starr DP (2000). Blood: An Epic History of Medicine and Commerce. New York: Quill. ISBN 0-688-17649-6.
^ Hirsch Jr ED (1991). What Your 1st Grader Needs to Know: Fundamentals of a Good First-Grade Education. pp 232–233. New York: Doubleday. ISBN 9780385411172.
^ "AB Plasma Donor Program". NIH Clinical Center. March 20, 2008. Archived from the original on February 15, 2022. Retrieved March 18, 2011.
^ Barclay L (October 23, 2007). "Female Plasma May Not Increase Risk for Transfusion-Related Acute Lung Injury". Medscape. Archived from the original on February 12, 2022. Retrieved July 2, 2011.
^ Roos R (December 19, 2003). "Blood transmission of vCJD suspected in Britain". Center for Infectious Disease Research and Policy. Archived from the original on June 24, 2021. Retrieved June 24, 2021.
^ "NHS pays £50m for US blood plasma firm". The Guardian. December 17, 2002. Archived from the original on June 24, 2021. Retrieved June 24, 2021.
^ Rankin J (July 18, 2013). "Bain Capital buys majority stake in Plasma Resources UK". The Guardian. Archived from the original on November 13, 2020. Retrieved June 24, 2021.
^ "Importation of plasma and use of apheresis platelets as risk reduction measures for variant Creutzfeldt-Jakob Disease" (PDF). Advisory Committee on the Safety of Blood, Tissues and Organs (SaBTO). March 2019. Archived (PDF) from the original on July 25, 2021. Retrieved June 24, 2021.
^ "Focus on plasma". blood.co.uk. January 2024. Archived from the original on January 7, 2024. Retrieved January 7, 2024.
^ Baino, F.; Yamaguchi, S. (December 2020). "The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges". Biomimetics. 5 (4). Biomimetics: 57. doi:10.3390/biomimetics5040057. PMC 7709622. PMID 33138246.

[1] Dennis O'Neil (1999). "Blood Components". Palomar College. Archived from the original on June 5, 2013.

[2] Tuskegee University (May 29, 2013). "Chapter 9 Blood". tuskegee.edu. Archived from the original on December 28, 2013.

[3] Mathew, Joscilin; Sankar, Parvathy; varacallo, Matthew (2024). "Physiology, blood plasma". Europe PMC. PMID 30285399. Retrieved January 23, 2024.

[Bloodplasma-4] "Ways to Keep Your Blood Plasma Healthy". BloodBanker. Archived from the original on November 1, 2013. Retrieved November 10, 2011.

[Maton-5] Maton A, Hopkins J, McLaughlin CW, Johnson S, Warner MQ, LaHart D, Wright JD (1993). Human Biology and Health. Englewood Cliffs, New Jersey: Prentice Hall. ISBN 0-13-981176-1.

[6] Tripathi S, Kumar V, Prabhakar A, Joshi S, Agrawal A (2015). "Passive blood plasma separation at the microscale: a review of design principles and microdevices". J. Micromech. Microeng. 25 (8): 083001. Bibcode:2015JMiMi..25h3001T. doi:10.1088/0960-1317/25/8/083001. S2CID 138153068.

[GuoHansson2020-7] Guo W, Hansson J, van der Wijngaart W (May 2020). "Synthetic Paper Separates Plasma from Whole Blood with Low Protein Loss". Analytical Chemistry. 92 (9): 6194–6199. doi:10.1021/acs.analchem.0c01474. PMID 32323979.

[8] Shmukler M (2004). Elert G (ed.). "Density of blood". The Physics Factbook. Archived from the original on December 9, 2021. Retrieved January 23, 2022.

[WHO2015E-9] "19th WHO Model List of Essential Medicines (April 2015)" (PDF). WHO. April 2015. Archived (PDF) from the original on April 28, 2019. Retrieved May 10, 2015.

[10] Soffer D (2008). "Usage of Blood Products in Multiple Casualty Incidents: The Experience of a Level I Trauma Center in Israel". Archives of Surgery. 143 (10): 983–989. doi:10.1001/archsurg.143.10.983. PMID 18936378. Retrieved January 23, 2024.

[11] Dargent A, Dumargne H, Labruyère M, et al. (October 2023). "Role of the interstitium during septic shock: a key to the understanding of fluid dynamics?". Intensive Care. 11 (44): 44. doi:10.1186/s40560-023-00694-z. PMC 10565984. PMID 37817235.

[12] Masoud M, Sarig G, Brenner B, Jacob G (June 2008). "Orthostatic hypercoagulability: a novel physiological mechanism to activate the coagulation system". Hypertension. 51 (6): 1545–1551. doi:10.1161/HYPERTENSIONAHA.108.112003. PMID 18413485.

[13] "Albumin: Liver Function Test - Viral Hepatitis and Liver Disease". U.S. Department of Veterans Affairs. Archived from the original on September 13, 2021. Retrieved March 15, 2021.

[14] "Globulins | Encyclopedia.com". www.encyclopedia.com. Archived from the original on November 29, 2021. Retrieved November 29, 2021.

[15] Thomas L (October 10, 2018). Simmons H (ed.). "Blood Plasma Components and Function". News-Medical.net. Archived from the original on November 29, 2021. Retrieved November 29, 2021.

[16] Biga LM, Dawson S, Harwell A, Hopkins R, Kaufmann J, LeMaster M, et al. (September 26, 2019). "18.1 Functions of Blood". Anatomy & Physiology. OpenStax. Archived from the original on November 29, 2021. Retrieved November 29, 2021 – via Oregon State University.

[17] "Blood cells". Basic Biology. 2015. Archived from the original on July 18, 2021. Retrieved March 17, 2020.

[18] Elkassabany NM, Meny GM, Doria RR, Marcucci C (April 2008). "Green plasma-revisited". Anesthesiology. 108 (4): 764–765. doi:10.1097/ALN.0b013e3181672668. PMID 18362615. Archived from the original on July 27, 2020. Retrieved March 21, 2020.

[19] Mani A, Poornima AP, Gupta D (2019). "Greenish discoloration of plasma: Is it really a matter of concern?". Asian Journal of Transfusion Science. 13 (1): 1–2. doi:10.4103/ajts.AJTS_117_18. PMC 6580839. PMID 31360002.

[20] Tesfazghi MT, McGill MR, Yarbrough ML (July 2019). "What's Causing This Dark Brown Plasma?". The Journal of Applied Laboratory Medicine. 4 (1): 125–129. doi:10.1373/jalm.2018.026633. PMID 31639715.

[21] Agnihotri N, Kumar L (July 2014). "Turbid plasma donations: Need for quantification". Asian Journal of Transfusion Science. 8 (2): 78–79. doi:10.4103/0973-6247.137436. PMC 4140067. PMID 25161342.

[:0-22] ^ ^a ^b ^c ^d ^e ^f "Use of anticoagulants in diagnostic laboratory investigations". World Health Organization. 2002. hdl:10665/65957. WHO/DIL/LAB/99.1 Rev.2.

[23] Jimenez Vera E, Chew YV, Nicholson L, Burns H, Anderson P, Chen HT, et al. (2019). "Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients". PLOS ONE. 14 (5): e0217163. Bibcode:2019PLoSO..1417163J. doi:10.1371/journal.pone.0217163. PMC 6530858. PMID 31116766.

[24] Kocijancic M, Cargonja J, Delic-Knezevic A (2014). "Evaluation of the BD Vacutainer(®) RST blood collection tube for routine chemistry analytes: clinical significance of differences and stability study". Biochemia Medica. 24 (3): 368–375. doi:10.11613/BM.2014.039. PMC 4210257. PMID 25351355.

[:1-25] Uges DR (October 1988). "Plasma or serum in therapeutic drug monitoring and clinical toxicology". Pharmaceutisch Weekblad. Scientific Edition. 10 (5): 185–188. doi:10.1007/BF01956868. PMID 3060834. S2CID 32330414.

[26] Wintrobe MM. Blood, Pure and Eloquent: a story of discovery, of people, and of ideas.

[27] Brake, Marisa; Ivanciu, Lacramioara; Maroney, Susan; Martinez, Nicolas; Mast, Alan; Westrick, Randal (March 15, 2019). "Assessing blood clotting and coagulation factors in mice". Current Protocols in Mouse Biology. 9 (2): e61. doi:10.1002/cpmo.61. PMC 6771260. PMID 30875463.

[28] Pusateri, Anthony; Given, Michael; Schreiber, Martin; Spinella, Philip; Pati, Shibani; Kozar, Rosemary; Khan, Abdul; Dacorta, Joseph; Kupferer, Kevin; Prat, Nicolas; Pidcoke, Heather; Macdonald, Victor; Malloy, Wilbur; Sailliol, Anne; Cap, Andrew (April 21, 2016). "Dried plasma: state of the science and recent developments". Transfusion. 56 (S2): S128–39. doi:10.1111/trf.13580. PMID 27100749. Retrieved January 23, 2024.

[:02-29] "When a Dream Comes True". grifols.com. January 2015. Archived from the original on July 25, 2021. Retrieved March 21, 2020.

[:12-30] "Biography: J.A. Grifols". discovertheplasma.com. Archived from the original on July 25, 2021. Retrieved March 21, 2020.

[31] "Father of the Blood Bank". Blood Cancer UK. Retrieved January 24, 2024.

[auto-32] "Home". achh.army.mil. Archived from the original on December 11, 2021. Retrieved December 9, 2021.

[plasma-33] Starr DP (2000). Blood: An Epic History of Medicine and Commerce. New York: Quill. ISBN 0-688-17649-6.

[34] Hirsch Jr ED (1991). What Your 1st Grader Needs to Know: Fundamentals of a Good First-Grade Education. pp 232–233. New York: Doubleday. ISBN 9780385411172.

[35] "AB Plasma Donor Program". NIH Clinical Center. March 20, 2008. Archived from the original on February 15, 2022. Retrieved March 18, 2011.

[36] Barclay L (October 23, 2007). "Female Plasma May Not Increase Risk for Transfusion-Related Acute Lung Injury". Medscape. Archived from the original on February 12, 2022. Retrieved July 2, 2011.

[37] Roos R (December 19, 2003). "Blood transmission of vCJD suspected in Britain". Center for Infectious Disease Research and Policy. Archived from the original on June 24, 2021. Retrieved June 24, 2021.

[38] "NHS pays £50m for US blood plasma firm". The Guardian. December 17, 2002. Archived from the original on June 24, 2021. Retrieved June 24, 2021.

[39] Rankin J (July 18, 2013). "Bain Capital buys majority stake in Plasma Resources UK". The Guardian. Archived from the original on November 13, 2020. Retrieved June 24, 2021.

[40] "Importation of plasma and use of apheresis platelets as risk reduction measures for variant Creutzfeldt-Jakob Disease" (PDF). Advisory Committee on the Safety of Blood, Tissues and Organs (SaBTO). March 2019. Archived (PDF) from the original on July 25, 2021. Retrieved June 24, 2021.

[41] "Focus on plasma". blood.co.uk. January 2024. Archived from the original on January 7, 2024. Retrieved January 7, 2024.

[42] Baino, F.; Yamaguchi, S. (December 2020). "The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges". Biomimetics. 5 (4). Biomimetics: 57. doi:10.3390/biomimetics5040057. PMC 7709622. PMID 33138246.

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 [[File:FreshFrozenPlasma.JPG|thumb|A unit of donated [[fresh plasma]]]]
-'''Blood plasma''' is a light [[Amber (color)|amber]]-colored liquid component of [[blood]] in which [[blood cell]]s are absent, however contains [[Blood protein|proteins]] and other constituents of [[whole blood]] in [[Suspension (chemistry)|suspension]]. It makes up about 55% of the body's total blood volume.<ref>{{cite web | url =https://anthro.palomar.edu/blood/blood_components.htm | title =Blood Components | author =Dennis O'Neil | publisher =[[Palomar College]] | year =1999 | url-status =dead | archive-url =https://web.archive.org/web/20130605052544/https://anthro.palomar.edu/blood/blood_components.htm | archive-date =June 5, 2013 }}</ref> It is the [[Intravascular compartment|intravascular part]] of [[extracellular fluid]] (all body fluid outside cells). It is mostly water (up to 95% by volume), and contains important dissolved proteins (6–8%; e.g., [[serum albumin]]s, [[globulin]]s, and [[fibrinogen]]),<ref>{{cite web | url =https://compepid.tuskegee.edu/syllabi/biomedical/Anatomy/microanat309/chapter9.htm | title =Chapter 9 Blood | author =Tuskegee University | author-link =Tuskegee University | publisher =tuskegee.edu | date =May 29, 2013 | url-status =dead | archive-url =https://archive.today/20131228034243/https://compepid.tuskegee.edu/syllabi/biomedical/Anatomy/microanat309/chapter9.htm | archive-date =December 28, 2013 }}</ref> [[glucose]], [[clotting factors]], [[electrolyte]]s ({{chem|Na|+}}, {{chem|Ca|2+}}, {{chem|Mg|2+}}, {{chem2|HCO_{3}^{−} }}, {{chem|Cl|−}}, etc.), [[hormone]]s, [[carbon dioxide]] (plasma being the main medium for excretory product transportation), and [[oxygen]] <ref>https://europepmc.org/article/nbk/nbk531504</ref>. It plays a vital role in an intravascular [[osmotic]] effect that keeps electrolyte concentration balanced and protects the body from [[infection]] and other blood-related disorders.<ref name=Bloodplasma>{{cite web |url=https://bloodbanker.com/plasma/plasma-donation/ways-to-keep-your-blood-plasma-healthy/ |title=Ways to Keep Your Blood Plasma Healthy | work = BloodBanker |access-date=November 10, 2011 |archive-url= https://web.archive.org/web/20131101022142/https://bloodbanker.com/plasma/plasma-donation/ways-to-keep-your-blood-plasma-healthy/ |archive-date=November 1, 2013 |url-status=dead}}</ref>
+'''Blood plasma''' is a light [[Amber (color)|amber]]-colored liquid component of [[blood]] in which [[blood cell]]s are absent, but which contains [[Blood protein|proteins]] and other constituents of [[whole blood]] in [[Suspension (chemistry)|suspension]]. It makes up about 55% of the body's total blood volume.<ref>{{cite web | url =https://anthro.palomar.edu/blood/blood_components.htm | title =Blood Components | author =Dennis O'Neil | publisher =[[Palomar College]] | year =1999 | url-status =dead | archive-url =https://web.archive.org/web/20130605052544/https://anthro.palomar.edu/blood/blood_components.htm | archive-date =June 5, 2013 }}</ref> It is the [[Intravascular compartment|intravascular part]] of [[extracellular fluid]] (all body fluid outside cells). It is mostly water (up to 95% by volume), and contains important dissolved proteins (6–8%; e.g., [[serum albumin]]s, [[globulin]]s, and [[fibrinogen]]),<ref>{{cite web | url =https://compepid.tuskegee.edu/syllabi/biomedical/Anatomy/microanat309/chapter9.htm | title =Chapter 9 Blood | author =Tuskegee University | author-link =Tuskegee University | publisher =tuskegee.edu | date =May 29, 2013 | url-status =dead | archive-url =https://archive.today/20131228034243/https://compepid.tuskegee.edu/syllabi/biomedical/Anatomy/microanat309/chapter9.htm | archive-date =December 28, 2013 }}</ref> [[glucose]], [[clotting factors]], [[electrolyte]]s ({{chem|Na|+}}, {{chem|Ca|2+}}, {{chem|Mg|2+}}, {{chem2|HCO_{3}^{−} }}, {{chem|Cl|−}}, etc.), [[hormone]]s, [[carbon dioxide]] (plasma being the main medium for excretory product transportation), and [[oxygen]].<ref>{{cite journal |last1=Mathew |first1=Joscilin |last2=Sankar |first2=Parvathy |last3=varacallo |first3=Matthew |title=Physiology, blood plasma |url=https://europepmc.org/article/nbk/nbk531504 |website=Europe PMC |date=2024 |pmid=30285399 |access-date= January 23, 2024}}</ref> It plays a vital role in an intravascular [[osmotic]] effect that keeps electrolyte concentration balanced and protects the body from [[infection]] and other blood-related disorders.<ref name=Bloodplasma>{{cite web |url=https://bloodbanker.com/plasma/plasma-donation/ways-to-keep-your-blood-plasma-healthy/ |title=Ways to Keep Your Blood Plasma Healthy | work = BloodBanker |access-date=November 10, 2011 |archive-url= https://web.archive.org/web/20131101022142/https://bloodbanker.com/plasma/plasma-donation/ways-to-keep-your-blood-plasma-healthy/ |archive-date=November 1, 2013 |url-status=dead}}</ref>
+Blood plasma can be separated from whole blood through [[blood fractionation]], by adding an [[anticoagulant]] to a tube filled with blood, which is spun in a [[centrifuge]] until the blood cells fall to the bottom of the tube. The blood plasma is then poured or drawn off.<ref name="Maton">{{cite book | vauthors = Maton A, Hopkins J, McLaughlin CW, Johnson S, Warner MQ, LaHart D, Wright JD | title = Human Biology and Health | publisher = Prentice Hall | year = 1993 | location = Englewood Cliffs, New Jersey | url = https://archive.org/details/humanbiologyheal00scho | isbn = 0-13-981176-1 | url-access = registration }}</ref> For [[point-of-care testing]] applications, plasma can be extracted from whole blood via filtration<ref>
-Blood plasma is separated from the blood by [[Blood fractionation|spinning a vessel of fresh blood]] containing an [[anticoagulant]] in a [[centrifuge]] until the blood cells fall to the bottom of the tube. The blood plasma is then poured or drawn off.<ref name="Maton">{{cite book | vauthors = Maton A, Hopkins J, McLaughlin CW, Johnson S, Warner MQ, LaHart D, Wright JD | title = Human Biology and Health | publisher = Prentice Hall | year = 1993 | location = Englewood Cliffs, New Jersey, USA | url = https://archive.org/details/humanbiologyheal00scho | isbn = 0-13-981176-1 | url-access = registration }}</ref> For [[point-of-care testing]] applications, plasma can be extracted from whole blood via filtration<ref>
 {{Cite journal|vauthors= Tripathi S, Kumar V, Prabhakar A, Joshi S, Agrawal A |date=2015|title=Passive blood plasma separation at the microscale: a review of design principles and microdevices |journal=J. Micromech. Microeng. |volume=25|issue=8|pages=083001 |doi=10.1088/0960-1317/25/8/083001 |bibcode=2015JMiMi..25h3001T|s2cid=138153068 }}</ref> or via [[Agglutination (biology)|agglutination]]<ref name="GuoHansson2020">{{cite journal | vauthors = Guo W, Hansson J, van der Wijngaart W | title = Synthetic Paper Separates Plasma from Whole Blood with Low Protein Loss | journal = Analytical Chemistry | volume = 92 | issue = 9 | pages = 6194–6199 | date = May 2020 | pmid = 32323979 | doi = 10.1021/acs.analchem.0c01474 | doi-access = free }}</ref> to allow for rapid testing of specific biomarkers. Blood plasma has a density of approximately {{cvt|1025|kg/m3|g/ml}}.<ref>{{cite web | url=https://hypertextbook.com/facts/2004/MichaelShmukler.shtml | title=Density of blood | vauthors = Shmukler M | year=2004 | website=The Physics Factbook | veditors = Elert G | access-date=2022-01-23 | archive-date=December 9, 2021 | archive-url=https://web.archive.org/web/20211209152325/https://hypertextbook.com/facts/2004/MichaelShmukler.shtml | url-status=live }}</ref> [[Serum (blood)|Blood serum]] is blood plasma without clotting factors.<ref name="Maton" /> [[Plasmapheresis]] is a medical therapy that involves blood plasma extraction, treatment, and reintegration.
 <!-- History, society and culture -->
-[[Fresh frozen plasma]] is on the [[WHO Model List of Essential Medicines]], the most important medications needed in a basic [[health system]].<ref name=WHO2015E>{{cite web |url=https://www.who.int/medicines/publications/essentialmedicines/EML2015_8-May-15.pdf |title=19th WHO Model List of Essential Medicines (April 2015) |date=April 2015 |access-date=May 10, 2015 |publisher=WHO |archive-date=April 28, 2019 |archive-url=https://web.archive.org/web/20190428215425/https://www.who.int/medicines/publications/essentialmedicines/EML2015_8-May-15.pdf |url-status=live }}</ref> It is of critical importance in the treatment of many types of trauma which result in blood loss, and is therefore kept stocked universally in all medical facilities capable of treating trauma (e.g., [[trauma center]]s, hospitals, and ambulances) or that pose a risk of patient blood loss such as surgical suite facilities  {{fact|date=September 2023}}.
+[[Fresh frozen plasma]] is on the [[WHO Model List of Essential Medicines]], the most important medications needed in a basic [[health system]].<ref name=WHO2015E>{{cite web |url=https://www.who.int/medicines/publications/essentialmedicines/EML2015_8-May-15.pdf |title=19th WHO Model List of Essential Medicines (April 2015) |date=April 2015 |access-date=May 10, 2015 |publisher=WHO |archive-date=April 28, 2019 |archive-url=https://web.archive.org/web/20190428215425/https://www.who.int/medicines/publications/essentialmedicines/EML2015_8-May-15.pdf |url-status=live }}</ref> It is of critical importance in the treatment of many types of trauma which result in blood loss, and is therefore kept stocked universally in all medical facilities capable of treating trauma (e.g., [[trauma center]]s, hospitals, and ambulances) or that pose a risk of patient blood loss such as surgical suite facilities <ref>{{cite journal | url=https://jamanetwork.com/journals/jamasurgery/article-abstract/401959 | title=Usage of Blood Products in Multiple Casualty Incidents: The Experience of a Level I Trauma Center in Israel | vauthors = Soffer D | year=2008 | journal=Archives of Surgery | volume=143 | issue=10 | pages=983–989 | doi=10.1001/archsurg.143.10.983 | pmid=18936378 | access-date=2024-01-23 }}</ref>
 ==Volume==
 {{multiple image|align=left|direction=vertical
 | image1 = Reference ranges for blood tests - by mass.svg| width1 = 550
-| caption1 = [[Reference ranges for blood tests]], showing normal mass concentration of blood plasma constituents.
+| caption1 = [[Reference ranges for blood tests]], showing normal mass concentration of blood plasma constituents
 | image2 = Reference ranges for blood tests - by molarity.png| width2 = 550
-| caption2 = The same information, shown in molarity rather than mass.
+| caption2 = The same information, shown in molarity rather than mass
 }}
-Blood plasma volume may be expanded by or drained to [[extravascular fluid]] when there are changes in [[Starling forces]] across capillary walls. For example, when [[blood pressure]] drops in [[Shock (circulatory)|circulatory shock]], Starling forces drive fluid into the [[interstitium]], causing [[Fluid compartments#Third spacing|third spacing]].{{cn|date=December 2021}}
+Blood plasma volume may be expanded by or drained to [[extravascular fluid]] when there are changes in [[Starling forces]] across capillary walls. For example, when [[blood pressure]] drops in [[Shock (circulatory)|circulatory shock]], Starling forces drive fluid into the [[interstitium]], causing [[Fluid compartments#Third spacing|third spacing]].<ref>{{cite journal | vauthors = Dargent A, Dumargne H, Labruyère M, et al | title = Role of the interstitium during septic shock: a key to the understanding of fluid dynamics? | journal = Intensive Care | volume = 11 | issue = 44 | date = October 2023 | page = 44 | doi = 10.1186/s40560-023-00694-z | doi-access = free| pmid = 37817235 | pmc = 10565984 }}</ref>
 Standing still for a prolonged period will cause an increase in [[transcapillary hydrostatic pressure]]. As a result, approximately 12% of blood plasma volume will cross into the [[extravascular compartment]]. This '''plasma shift''' causes an increase in [[hematocrit]], [[serum total protein]], [[blood viscosity]] and, as a result of increased concentration of [[coagulation factors]], it causes [[orthostatic hypercoagulability]].<ref>{{cite journal | vauthors = Masoud M, Sarig G, Brenner B, Jacob G | title = Orthostatic hypercoagulability: a novel physiological mechanism to activate the coagulation system | journal = Hypertension | volume = 51 | issue = 6 | pages = 1545–1551 | date = June 2008 | pmid = 18413485 | doi = 10.1161/HYPERTENSIONAHA.108.112003 | doi-access = free }}</ref>
@@ Line 45: / Line 43: @@
 == Plasma vs. serum in medical diagnostics ==
-Blood plasma and [[blood serum]] are often used in [[blood test]]s. Some tests can be done only on plasma and some only on serum. Some can be done on both, but depending on the test, use of either plasma or serum can be more practical.<ref name=":0" /> In addition, some tests have to be done with [[whole blood]], such as the determination of the amount of [[blood cell]]s in blood via [[flow cytometry]].<ref>{{cite journal | vauthors = Jimenez Vera E, Chew YV, Nicholson L, Burns H, Anderson P, Chen HT, Williams L, Keung K, Zanjani NT, Dervish S, Patrick E, Wang XM, Yi S, Hawthorne W, Alexander S, O'Connell PJ, Hu M | display-authors = 6 | title = Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients | journal = PLOS ONE | volume = 14 | issue = 5 | pages = e0217163 | date = 2019 | pmid = 31116766 | pmc = 6530858 | doi = 10.1371/journal.pone.0217163 | doi-access = free | bibcode = 2019PLoSO..1417163J }}</ref>
+Blood plasma and [[blood serum]] are often used in [[blood test]]s. Tests can be done on plasma, serum or both.<ref name=":0" /> In addition, some tests have to be done with [[whole blood]], such as the determination of the amount of [[blood cell]]s in blood via [[flow cytometry]].<ref>{{cite journal | vauthors = Jimenez Vera E, Chew YV, Nicholson L, Burns H, Anderson P, Chen HT, Williams L, Keung K, Zanjani NT, Dervish S, Patrick E, Wang XM, Yi S, Hawthorne W, Alexander S, O'Connell PJ, Hu M | display-authors = 6 | title = Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients | journal = PLOS ONE | volume = 14 | issue = 5 | pages = e0217163 | date = 2019 | pmid = 31116766 | pmc = 6530858 | doi = 10.1371/journal.pone.0217163 | doi-access = free | bibcode = 2019PLoSO..1417163J }}</ref>
 {| class="wikitable"
 |+
@@ Line 66: / Line 64: @@
 ==History==
 [[File:Private Roy W. Humphrey of Toledo, Ohio is being given blood plasma after he was wounded by shrapnel in Sicily on 8-9-43 - NARA - 197268.jpg|thumb|Private Roy W. Humphrey is being given blood plasma after he was wounded by shrapnel in Sicily in August 1943.]]
-[[Image:Britain and us plasma packages wwii.jpg|thumb|Dried plasma packages used by the British and US militaries during WWII.]]
+[[Image:Britain and us plasma packages wwii.jpg|thumb|Dried plasma packages used by the British and US militaries during WWII]]
-Plasma was already well known when described by William Harvey in ''de Mortu Cordis'' in 1628, but knowledge of it probably dates as far back as Vesalius (1514–1564). The discovery of fibrinogen by William Henson, {{Circa|1770}},<ref>{{cite book| vauthors = Wintrobe MM |title=Blood, Pure and Eloquent: a story of discovery, of people, and of ideas }}</ref> made it easier to study plasma, as ordinarily, upon coming in contact with a foreign surface – something other than the vascular endothelium – clotting factors become activated and clotting proceeds rapidly, trapping RBCs etc. in the plasma and preventing separation of plasma from the blood. Adding citrate and other anticoagulants is a relatively recent advance. Upon the formation of a clot, the remaining clear fluid (if any) is blood serum, which is essentially plasma without the clotting factors.{{cn|date=December 2021}}
+Plasma was already well known when described by William Harvey in ''de Motu Cordis'' in 1628, but knowledge of it probably dates as far back as Vesalius (1514–1564). The discovery of fibrinogen by William Henson, {{Circa|1770}},<ref>{{cite book| vauthors = Wintrobe MM |title=Blood, Pure and Eloquent: a story of discovery, of people, and of ideas }}</ref> made it easier to study plasma, as ordinarily, upon coming in contact with a foreign surface – something other than the vascular endothelium – clotting factors become activated and clotting proceeds rapidly, trapping RBCs etc. in the plasma and preventing separation of plasma from the blood. Adding citrate and other anticoagulants is a relatively recent advance. Upon the formation of a clot, the remaining clear fluid (if any) is blood serum, which is essentially plasma without the clotting factors<ref>{{cite journal |last1=Brake |first1=Marisa |last2=Ivanciu |first2=Lacramioara |last3=Maroney |first3=Susan |last4=Martinez |first4=Nicolas |last5=Mast |first5=Alan |last6=Westrick |first6=Randal |title=Assessing blood clotting and coagulation factors in mice |journal=Current Protocols in Mouse Biology |date=March 15, 2019 |volume=9 |issue=2 |page=e61 |doi=10.1002/cpmo.61 |pmid=30875463 |pmc=6771260 }}</ref>
-The use of blood plasma as a substitute for whole blood and for transfusion purposes was proposed in March 1918, in the correspondence columns of the British Medical Journal, by Gordon R. Ward. "Dried plasmas" in powder or strips of material format were developed and first used in [[World War II]]. Prior to the United States' involvement in the war, liquid plasma and [[whole blood]] were used.{{cn|date=December 2021}}
+The use of blood plasma as a substitute for whole blood and for transfusion purposes was proposed in March 1918, in the correspondence columns of the British Medical Journal, by Gordon R. Ward. "Dried plasmas" in powder or strips of material format were developed and first used in [[World War II]]. Prior to the United States' involvement in the war, liquid plasma and [[whole blood]] were used.<ref>{{cite journal |last1=Pusateri |first1=Anthony |last2=Given |first2=Michael |last3=Schreiber |first3=Martin |last4=Spinella |first4=Philip |last5=Pati |first5=Shibani |last6=Kozar |first6=Rosemary |last7=Khan |first7=Abdul |last8=Dacorta |first8=Joseph |last9=Kupferer |first9=Kevin |last10=Prat |first10=Nicolas |last11=Pidcoke |first11=Heather |last12=Macdonald |first12=Victor |last13=Malloy |first13=Wilbur |last14=Sailliol |first14=Anne |last15=Cap |first15=Andrew |title=Dried plasma: state of the science and recent developments |journal=Transfusion |date=April 21, 2016 |volume=56 |issue=S2 |pages=S128–39 |doi=10.1111/trf.13580 |pmid=27100749 |url=https://onlinelibrary.wiley.com/doi/full/10.1111/trf.13580 |access-date=January 23, 2024}}</ref>
 === The origin of plasmapheresis ===
-Dr. José Antonio Grifols Lucas, a scientist from Vilanova i la Geltrú, Spain,<ref name=":02">{{Cite web|url=https://www.grifols.com/documents/51507592/51526363/when-a-dream-comes-true.pdf/c0ebb294-4db1-4826-95b6-e9468aa9305c|title=When a Dream Comes True|date=January 2015|website=grifols.com|access-date=March 21, 2020|archive-date=July 25, 2021|archive-url=https://web.archive.org/web/20210725084332/https://www.grifols.com/documents/51507592/51526363/when-a-dream-comes-true.pdf/c0ebb294-4db1-4826-95b6-e9468aa9305c|url-status=live}}</ref> founded Laboratorios Grifols in 1940.<ref name=":12">{{Cite web|url=https://www.discovertheplasma.com/documents/15447917/15449136/biography_ja_grifols.pdf/036f08c5-630b-4e4b-b590-985b4e951495|title=Biography: J.A. Grifols|website=discovertheplasma.com|access-date=March 21, 2020|archive-date=July 25, 2021|archive-url=https://web.archive.org/web/20210725085451/https://www.discovertheplasma.com/documents/15447917/15449136/biography_ja_grifols.pdf/036f08c5-630b-4e4b-b590-985b4e951495|url-status=live}}</ref> Dr. Grifols pioneered a first-of-its-kind technique called ''plasmapheresis'',<ref name=":12" /> where a donor's red blood cells would be returned to the donor's body almost immediately after the separation of the blood plasma. This technique is still in practice today, almost 80 years later. In 1945, Dr. Grifols opened the world's first plasma donation center.<ref name=":02" /> Thirteen years after the center's opening, Dr. Grifols unexpectedly died at the young age of 41 due to leukemia.
+Dr. José Antonio Grifols Lucas, a scientist from Vilanova i la Geltrú, Spain,<ref name=":02">{{Cite web|url=https://www.grifols.com/documents/51507592/51526363/when-a-dream-comes-true.pdf/c0ebb294-4db1-4826-95b6-e9468aa9305c|title=When a Dream Comes True|date=January 2015|website=grifols.com|access-date=March 21, 2020|archive-date=July 25, 2021|archive-url=https://web.archive.org/web/20210725084332/https://www.grifols.com/documents/51507592/51526363/when-a-dream-comes-true.pdf/c0ebb294-4db1-4826-95b6-e9468aa9305c|url-status=live}}</ref> founded Laboratorios Grifols in 1940.<ref name=":12">{{Cite web|url=https://www.discovertheplasma.com/documents/15447917/15449136/biography_ja_grifols.pdf/036f08c5-630b-4e4b-b590-985b4e951495|title=Biography: J.A. Grifols|website=discovertheplasma.com|access-date=March 21, 2020|archive-date=July 25, 2021|archive-url=https://web.archive.org/web/20210725085451/https://www.discovertheplasma.com/documents/15447917/15449136/biography_ja_grifols.pdf/036f08c5-630b-4e4b-b590-985b4e951495|url-status=live}}</ref> Dr. Grifols pioneered a first-of-its-kind technique called ''plasmapheresis'',<ref name=":12" /> where a donor's red blood cells would be returned to the donor's body almost immediately after the separation of the blood plasma. This technique is still in practice today, almost 80 years later. In 1945, Dr. Grifols opened the world's first plasma donation center.<ref name=":02" />
 ===Blood for Britain===  <!-- subject of redirect -->
-The "Blood for Britain" program during the early 1940s was quite successful (and popular in the United States) based on [[Charles R. Drew|Charles Drew]]'s contribution. A large project began in August 1940 to collect blood in New York City hospitals for the export of plasma to Britain. Drew was appointed medical supervisor of the "[[Plasma for Britain]]" project. His notable contribution at this time was to transform the [[test tube]] methods of many blood researchers into the first successful mass production techniques.{{cn|date=December 2021}}
+The "Blood for Britain" program during the early 1940s was quite successful (and popular in the United States) based on [[Charles R. Drew|Charles Drew]]'s contribution. A large project began in August 1940 to collect blood in New York City hospitals for the export of plasma to Britain. Drew was appointed medical supervisor of the "[[Plasma for Britain]]" project. His notable contribution at this time was to transform the [[test tube]] methods of many blood researchers into the first successful mass production techniques.<ref>{{Cite web|url=https://bloodcancer.org.uk/news/charles-r-drew-the-father-of-the-blood-bank/#:~:text=Blood%20For%20Britain&text=Drew%20was%20headhunted%20by%20British,Britain%20by%20the%20Red%20Cross|title=Father of the Blood Bank|website=Blood Cancer UK|access-date=January 24, 2024}}</ref>
-Nevertheless, the decision was made to develop a dried plasma package for the armed forces as it would reduce breakage and make the transportation, packaging, and storage much simpler.<ref name="auto">{{Cite web|url=https://achh.army.mil/|title=Home|website=achh.army.mil|access-date=December 9, 2021|archive-date=December 11, 2021|archive-url=https://web.archive.org/web/20211211061644/https://achh.army.mil/|url-status=live}}</ref> The resulting dried plasma package came in two tin cans containing 400 cc bottles. One bottle contained enough [[distilled water]] to reconstitute the dried plasma contained within the other bottle. In about three minutes, the plasma would be ready to use and could stay fresh for around four hours.<ref name="auto"/> The Blood for Britain program operated successfully for five months, with total collections of almost 15,000 people donating blood, and with over 5,500 vials of blood plasma.<ref name="plasma">{{cite book | vauthors = Starr DP | title = Blood: An Epic History of Medicine and Commerce | location = New York | publisher = Quill | year = 2000 | isbn = 0-688-17649-6 }}</ref>
+Nevertheless, the decision was made to develop a dried plasma package for the armed forces as it would reduce breakage and make the transportation, packaging, and storage much simpler.<ref name="auto">{{Cite web|url=https://achh.army.mil/|title=Home|website=achh.army.mil|access-date=December 9, 2021|archive-date=December 11, 2021|archive-url=https://web.archive.org/web/20211211061644/https://achh.army.mil/|url-status=live}}</ref> The resulting dried plasma package came in two tin cans containing 400 cc bottles. One bottle contained enough [[distilled water]] to reconstitute the dried plasma contained within the other bottle. In about three minutes, the plasma would be ready to use and could stay fresh for around four hours. The Blood for Britain program operated successfully for five months, with total collections of almost 15,000 people donating blood, and with over 5,500 vials of blood plasma.<ref name="plasma">{{cite book | vauthors = Starr DP | title = Blood: An Epic History of Medicine and Commerce | location = New York | publisher = Quill | year = 2000 | isbn = 0-688-17649-6 }}</ref>
 Following the Supplying Blood Plasma to England project, Drew was named director of the [[Red Cross]] [[blood bank]] and assistant director of the [[United States National Research Council|National Research Council]], in charge of blood collection for the [[United States Army]] and [[United States Navy|Navy]]. Drew argued against the armed forces directive that blood/plasma was to be separated by the [[Race (human classification)|race]] of the [[blood donation|donor]]. Drew insisted that there was no racial difference in human blood and that the policy would lead to needless deaths as soldiers and sailors were required to wait for "same race" blood.<ref>{{cite book| vauthors = Hirsch Jr ED |author-link=Eric Donald Hirsch Jr.|title=What Your 1st Grader Needs to Know: Fundamentals of a Good First-Grade Education|url=https://archive.org/details/whatyour1stgrade00edhi|url-access=registration|publisher=New York: Doubleday|series=pp 232–233|year=1991|isbn=9780385411172}}</ref>
@@ Line 90: / Line 88: @@
 Following fears of [[variant Creutzfeldt-Jakob disease]] ([[vCJD]]) being spread through the blood supply, the British government began to phase out blood plasma from U.K. donors and by the end of 1999 had imported all blood products made with plasma from the United States.<ref>{{cite web | vauthors = Roos R |title=Blood transmission of vCJD suspected in Britain |url=https://www.cidrap.umn.edu/news-perspective/2003/12/blood-transmission-vcjd-suspected-britain |website=Center for Infectious Disease Research and Policy |date=December 19, 2003 |access-date=24 June 2021 |archive-date=June 24, 2021 |archive-url=https://web.archive.org/web/20210624205508/https://www.cidrap.umn.edu/news-perspective/2003/12/blood-transmission-vcjd-suspected-britain |url-status=live }}</ref> In 2002, the British government purchased Life Resources Incorporated, an American blood supply company, to import plasma.<ref>{{cite web |title=NHS pays £50m for US blood plasma firm |url=https://www.theguardian.com/society/2002/dec/17/bse.medicineandhealth |website=The Guardian |date=December 17, 2002 |access-date=24 June 2021 |archive-date=June 24, 2021 |archive-url=https://web.archive.org/web/20210624205220/https://www.theguardian.com/society/2002/dec/17/bse.medicineandhealth |url-status=live }}</ref> The company became Plasma Resources UK (PRUK) which owned [[Bio Products Laboratory]]. In 2013, the British government sold an 80% stake in PRUK to American hedge fund [[Bain Capital]], in a deal estimated to be worth £200 million. The sale was met with criticism in the UK.<ref>{{cite web | vauthors = Rankin J |title=Bain Capital buys majority stake in Plasma Resources UK |url=https://www.theguardian.com/business/2013/jul/18/bain-capital-plasma-resources-uk |website=The Guardian |date=July 18, 2013 |access-date=24 June 2021 |archive-date=November 13, 2020 |archive-url=https://web.archive.org/web/20201113070906/https://www.theguardian.com/business/2013/jul/18/bain-capital-plasma-resources-uk |url-status=live }}</ref> In 2009, the U.K. stopped importing plasma from the United States, as it was no longer a viable option due to regulatory and jurisdictional challenges.<ref>{{cite web |title=Importation of plasma and use of apheresis platelets as risk reduction measures for variant Creutzfeldt-Jakob Disease | work = Advisory Committee on the Safety of Blood, Tissues and Organs (SaBTO) | date = March 2019 |url=https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/829906/SaBTO_PC_report.pdf |access-date=24 June 2021 |archive-date=July 25, 2021 |archive-url=https://web.archive.org/web/20210725084726/https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/829906/SaBTO_PC_report.pdf |url-status=live }}</ref>
-Currently,{{when|date=March 2020}} blood donated in the United Kingdom is used by UK Blood Services for the manufacture of plasma blood components (Fresh Frozen Plasma (FFP) and cryoprecipitate). However, plasma from UK donors is still not used for the commercial manufacture of fractionated plasma medicines.<ref>{{cite web |title=Focus on plasma | publisher = blood.co.uk | date = January 2024 |url=https://www.blood.co.uk/news-and-campaigns/the-donor/focus-on-plasma/ |access-date=7 January 2024 |archive-date=January 7, 2024 |archive-url=https://web.archive.org/web/20240107011115/https://www.blood.co.uk/news-and-campaigns/the-donor/focus-on-plasma/ |url-status=live }}</ref>
+At present (2024), blood donated in the United Kingdom is used by UK Blood Services for the manufacture of plasma blood components (Fresh Frozen Plasma (FFP) and cryoprecipitate). However, plasma from UK donors is still not used for the commercial manufacture of fractionated plasma medicines.<ref>{{cite web |title=Focus on plasma | publisher = blood.co.uk | date = January 2024 |url=https://www.blood.co.uk/news-and-campaigns/the-donor/focus-on-plasma/ |access-date=7 January 2024 |archive-date=January 7, 2024 |archive-url=https://web.archive.org/web/20240107011115/https://www.blood.co.uk/news-and-campaigns/the-donor/focus-on-plasma/ |url-status=live }}</ref>
 ==Synthetic blood plasma==
-[[Simulated body fluid]] (SBF) is a solution having a similar ion concentration to that of human blood plasma. SBF is normally used for the surface modification of metallic implants, and more recently in [[gene delivery]] application {{fact|date=September 2023}}.
+[[Simulated body fluid]] (SBF) is a solution having a similar ion concentration to that of human blood plasma. SBF is normally used for the surface modification of metallic implants, and more recently in [[gene delivery]] application.<ref>{{cite journal |title=The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges|publisher=Biomimetics |date=December 2020 |pmc=7709622 |last1=Baino |first1=F. |last2=Yamaguchi |first2=S. |journal=Biomimetics |volume=5 |issue=4 |page=57 |doi=10.3390/biomimetics5040057 |doi-access=free |pmid=33138246 }}</ref>
 == See also ==

v t e Blood transfusion and transfusion medicine
Blood products	Whole blood Platelets Platelet transfusion Red blood cells Plasma Fresh frozen plasma PF24 Cryoprecipitate Cryosupernatant White blood cells Granulocyte transfusion Blood substitutes
General concepts	Blood bank Blood donation Blood management International Society of Blood Transfusion ISBT 128
Methods	Apheresis (plasmapheresis, plateletpheresis, leukapheresis) Exchange transfusion Intraoperative blood salvage
Tests	Blood compatibility testing Cross-matching Coombs test Kleihauer–Betke test Antibody elution Monocyte monolayer assay
Transfusion reactions and adverse effects	Transfusion hemosiderosis Transfusion related acute lung injury Transfusion associated circulatory overload Transfusion-associated graft versus host disease Febrile non-hemolytic transfusion reaction Hemolytic reaction acute delayed Serum sickness Transfusion transmitted infection
Blood group systems	Blood types ABO Secretor status Augustine CD59 Chido-Rodgers Colton Cromer Diego Dombrock Duffy Er FORS Gerbich GIL GLOB Hh Ii Indian JR JMH KANNO Kell (Xk) Kidd Knops Lan Lewis Lutheran LW MNS OK P1PK Raph Rh and RHAG Scianna Sid T-Tn Vel Xg Yt Other