DUI is a three letter acronym (or initialism) that may stand for:
- Driving under the influence (of alcohol or drugs)
- Democratic Union for Integration — the largest ethnic Albanian party in the Republic of Macedonia
- Data Use Identifier
- Data Use Institute
- Davis Unified Ignition * Diving Unlimited International Inc.
- Documento Único de Identidad — voting identification document in El Salvador
- Documento Unico de Importación
- Duke University Improv
- Distinctive unit insignia — a metal heraldic device worn by soldiers in the U.S. Army
- A shortname for the creator god Tuisto of the Celts
- Melvil Dewey tried to shorten his name to Melvil Dui following his proposals for English spelling reform.