Kiss-and-run fusion: Difference between revisions

}}</ref> MacDonald et al. used multiple approaches to test for kiss-and-run exocytosis in rat beta cells. By monitoring membrane patches of intact rat beta cells in the presence of 10 mM [[glucose]] and 5 mM [[forskolin]], MacDonald et al. found that some vesicles underwent kiss-and-run, as seen by an exocytotic event followed by an [[endocytotic]] event of a similar magnitude.<ref name="Braun" /> Kiss-and-run events accounted for 25% of LDCV exocytosis and 28% of SLV exocytosis.<ref name="Braun" /> Interestingly, whileWhile LDCV kiss-and-run occurred 25% of the time in the presence of forskolin, in the absence of forskolin, LDCV kiss-and-run fusion occurred only 7% of the time.<ref name="Braun" /> Because forskolin raises [[cyclic AMP]] (cAMP) levels, cAMP seemingly plays a very important role in the mechanism in LDCV kiss-and-run fusion in rat pancreatic beta cells.