File:Ultrasound-guided injection of the rete testis of a monkey.png
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Summary
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FIGURE 5 Ultrasound-guided injection of the rete testis (A) Saline solution with trypan blue (approximately 1 ml) was introduced into the recipient's seminiferous tubules through testicular rete injection. The rete testis of a 60-M-old rhesus monkey (arrowhead) is visible under ultrasonographic guidance. Videos are presented in our Supporting Data. (B) An injection needle (arrow) was used to puncture the rete testis, and the solution was gently injected. (C) Trypan blue-stained seminiferous tubules can be observed on the surface of the injected testis. (D) Bisection of the injected testis indicated that ultrasound-guided injection was not invasive. The lumen of the testicular seminiferous tubules was filled with trypan blue to approximately 70%– 80% of the testicular volume. (E and F) The presence of trypan blue solution in the lumen of seminiferous tubules was assessed through a stereoscope after dissociating testicular tubules in a culture dish, thus confirming successful intratubular injection (scale bars, 1 cm in C and D; 500 μm in E; and 200 μm in F) |
| Date | |
| Source | https://www.researchgate.net/publication/358272181_Primary_culture_of_germ_cells_that_portray_stem_cell_characteristics_and_recipient_preparation_for_autologous_transplantation_in_the_rhesus_monkey Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey. Journal of Cellular and Molecular Medicine 26(1), DOI:10.1111/jcmm.17197 |
| Author | Huaqin Yu an, Jiache n Sun, Sheng nan Wang, Ziyi Xi ang. |
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This is an op en access ar ticle under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
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| current | 18:18, 4 August 2024 | | 1,048 × 1,193 (1.1 MB) | Rasbak (talk | contribs) | {{Information |description=FIGURE 5 Ultrasound-guided injection of the rete testis (A) Saline solution with trypan blue (approximately 1 ml) was introduced into the recipient's seminiferous tubules through testicular rete injection. The rete testis of a 60-M-old rhesus monkey (arrowhead) is visible under ultrasonographic guidance. Videos are presented in our Supporting Data. (B) An injection needle (arrow) was used to puncture the rete testis, and the solution was gently injected. (C) Trypan... |
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