File:Function-specific haploinsufficiency phenotypes. Yeast.png

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Fig 2. Function-specific haploinsufficiency phenotypes.

(A) Images of heterozygous mutants for RNA pol II (RPC10/rpc10Δ), chaperonin CCT (CCT5/cct5Δ), and wild type. Cells were stained with FITC-conjugated concanavalin A for cell wall (green), rhodamine-phalloidin for actin (red), and DAPI for nuclear DNA (blue), and presented with pseudo-coloring. Scale bar indicates 10 μm. (B) Biased distribution of heterozygous mutants in phenotypic space. After CCA was performed, scores of CVs of the haploinsufficiency phenotypes were plotted in pCV1/pCV3 orthogonal space. Green and red circles indicate mutants heterozygous for RNA pol II and chaperonin CCT, respectively. Gray and yellow circles indicate the remaining heterozygotes and 114 wild-type replicates, respectively. (C) Linear predictor of logistic regression showing phenotype specificity for the cytosolic large ribosomal subunit (GO:0022625) heterozygous mutants (S3 Data). Linear predictor was calculated by linear combinations of 10 pCVs (pCV1, pCV2, pCV4, pCV5, pCV12, pCV13, pCV17, pCV18, pCV19, and pCV21) selected by a combinational optimization. Asterisk indicates significant difference (P < 0.05 after Bonferroni correction by likelihood ratio test). CCA, canonical correlation analysis; CCT, chaperonin containing TCP-1; DAPI, 4’,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; pCV, phenotype canonical variable; RNA pol II, RNA polymerase II core complex; RPL, ribosomal protein of the large subunit; WT, wild type.
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Source https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2005130 Ohnuki S, Ohya Y (2018) High-dimensional single-cell phenotyping reveals extensive haploinsufficiency. PLoS Biol 16(5): e2005130. https://doi.org/10.1371/journal.pbio.2005130
Author Ohnuki S, Ohya Y
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© 2018 Ohnuki, Ohya. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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current23:58, 29 September 2024Thumbnail for version as of 23:58, 29 September 20244,198 × 3,288 (1.9 MB)Rasbak (talk | contribs){{Information |description=Fig 2. Function-specific haploinsufficiency phenotypes.<br> (A) Images of heterozygous mutants for RNA pol II (RPC10/rpc10Δ), chaperonin CCT (CCT5/cct5Δ), and wild type. Cells were stained with FITC-conjugated concanavalin A for cell wall (green), rhodamine-phalloidin for actin (red), and DAPI for nuclear DNA (blue), and presented with pseudo-coloring. Scale bar indicates 10 μm. (B) Biased distribution of heterozygous mutants in phenotypic space. After CCA was perfo...

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