File:A-dynamin-1--dynamin-3--and-clathrin-independent-pathway-of-synaptic-vesicle-recycling-mediated-by-elife01621f005.jpg

English: (A) Control (Dyn3 KO) and Dyn1/3 DKO neurons were stimulated by a 90 s high K+ stimulation in the presence of HRP, rested for 30 min in the absence of HRP and then subjected to a second round of high K+ stimulation and recovery, always in the absence of HRP. Loss of HRP-labeled SVs during the second stimulus, and the subsequent recovery of unlabeled SVs, demonstrates that vesicles generated from bulk endosomes are functional SVs. (B) Quantification of the results shown in A. Scale bar = 500 nm. **** indicates p-values of <0.0001. Black asterisks refer to comparisons between total vesicles, colored asterisks to comparisons between HRP-labeled vesicles. Bars: standard error of the mean.